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METHOD FOR INTEGRALLY DETECTING NONDESTRUCTIVE MEASUREMENT INFORMATION AND GENOME-RELATED INFORMATION OF ONE CELL

  • US 20200131562A1
  • Filed: 05/02/2018
  • Published: 04/30/2020
  • Est. Priority Date: 05/02/2017
  • Status: Active Grant
First Claim
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1. A method for integrally detecting nondestructive measurement information and genome-related information of single cells, the method comprising:

  • preparing a plurality of compartments containing a single cell or a derivative thereof, a first bead(s), and a second bead(s) per compartment,wherein each first bead isa particle cleavably linked to a first barcode nucleic acid corresponding to each imaging information oran organism containing a first barcode nucleic acid corresponding to each imaging information, andimaging information of the first bead(s) in each compartment can be clearly distinguished from each other, andthe second bead(s) is linked to a plurality of second barcode nucleic acids hybridizable with a genome-related nucleic acid corresponding to a cell genome or an expressed product thereof or the first barcode nucleic acid;

    detecting both nondestructive measurement information of the single cell and imaging information of the first bead(s) and associating the nondestructive measurement information of the single cell with the imaging information of the first bead(s) before preparation of each compartment or in each compartment;

    cleaving the first barcode nucleic acid from the associated first bead(s), and hybridizing each of the genome-related nucleic acid and the first barcode nucleic acid with the second barcode nucleic acid to obtain a hybridized complex;

    producing an amplified product derived from the hybridized complex; and

    integrally detecting nondestructive measurement information and genome-related information in the single cell using an expression pattern of the amplified product as an index.

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