SENSING APPARATUS FOR AMPLIFICATION AND SEQUENCING OF TEMPLATE POLYNUCLEOTIDES AND ARRAY FOR AMPLIFICATION OF TEMPLATE POLYNUCLEOTIDES
First Claim
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1. An array for amplification and sequencing of template polynucleotides, the array comprising:
- a plurality of wells wherein each well is exposed to an ISFET;
a plurality of capture beads, wherein each capture bead comprises a single template binding site,wherein the wells and capture beads are relatively sized so that only one bead will fit into each well;
a removable seal arranged to cover the wells and isolate each well from adjacent wells; and
a heating element.
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Abstract
Provided is a sensing apparatus comprising a chip for integrated amplification and sequencing of a template polynucleotide in a sample. The apparatus comprises a chip with at least one ISFET in a well or chamber, amplification means for amplifying the template polynucleotide on a surface of said chip and comprising at least one heating means suitable for conducting amplification of the template polynucleotide at temperatures elevated with respect to room temperature, and sequencing means for sequencing the amplified template polynucleotide in said well or chamber. Methods of use are also provided.
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16 Claims
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1. An array for amplification and sequencing of template polynucleotides, the array comprising:
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a plurality of wells wherein each well is exposed to an ISFET; a plurality of capture beads, wherein each capture bead comprises a single template binding site, wherein the wells and capture beads are relatively sized so that only one bead will fit into each well; a removable seal arranged to cover the wells and isolate each well from adjacent wells; and a heating element. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification