METHODS AND MATERIALS FOR DETECTING COLORECTAL CANCER AND ADENOMA
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Abstract
The present invention provides methods and materials related to the detection of colorectal neoplasm-specific markers (e.g., markers associated with colorectal cancer, markers associated with adenoma) in or associated with a subject'"'"'s stool sample. In particular, the present invention provides methods and materials for identifying mammals (e.g., humans) having a colorectal neoplasm by detecting the presence and level of indicators of colorectal neoplasia such as, for example, long DNA (e.g., quantified by Alu PCR) and the presence and level of tumor-associated gene alterations (e.g., mutations in KRAS, APC, melanoma antigen gene, p53, BRAF, BAT26, PIK3CA) or epigenetic alterations (e.g., DNA methylation) (e.g., CpG methylation) (e.g., CpG methylation in coding or regulatory regions of bmp-3, bmp-4, SFRP2, vimentin, septin9, ALX4, EYA4, TFPI2, NDRG4, FOXE1) in DNA from a stool sample obtained from the mammal.
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4 Claims
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1. (canceled)
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2. A method, comprising:
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a) extracting genomic DNA from a biological sample of a human individual suspected of having or having colorectal neoplasia, b) treating the extracted genomic DNA with bisulfite, c) amplifying the bisulfite-treated genomic DNA with primers consisting of a pair of primers specific for BMP3 and a pair of primers specific for NDRG4, d) measuring the methylation level of one or more CpG sites in BMP3 and NDRG4, and e) performing a colonoscopy on the human individual if the measured methylation level of the one or more CpG sites in BMP3 and NDRG4 is higher than an established control methylation level for BMP3 and NDRG4 from human individuals not having colorectal neoplasia. - View Dependent Claims (3, 4)
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Specification