YEAST WITH IMPROVED ALCOHOL PRODUCTION

• US 20200131591A1
• Filed: 06/04/2018
• Published: 04/30/2020
• Est. Priority Date: 06/06/2017
• Status: Active Application

• Current Assignee: Danisco USA Incorporated (International Flavors & Fragrances Incorporated)
• Original Assignee: Danisco USA Incorporated (International Flavors & Fragrances Incorporated)
• Inventors: Paven, Celia Emily Gaby, Qi, Min
• Application Number:
  US16/619,565
• Publication Number:
  US 20200131591A1
• Time in Patent Office: Days
• CPC Class Codes: C12N 1/18 Baker's yeast; Brewer's yeast
• CPC Class Codes: C12N 1/185 Saccharomyces isolates
• CPC Class Codes: C12N 15/81 for yeasts
• CPC Class Codes: C12N 9/90 Isomerases (5.)
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: C12R 2001/85 Saccharomyces
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

Described are compositions and methods relating to yeast cells having a genetic mutation that give rise to increased alcohol production. Such yeast is well-suited for use in alcohol production to reduce fermentation time and/or increase yields.

Claims

1. Modified yeast cells derived from parental yeast cells, the modified cells comprising a genetic alteration that causes the modified cells to produce a decreased amount of functional Cpr1 polypeptide compared to the parental cells, wherein the modified cells produce during fermentation an increased amount of ethanol compared to parental cells under equivalent fermentation conditions.

2. The modified cells of claim 1, wherein the genetic alteration comprises a disruption of the YDR155c gene present in the parental cells.

3. The modified cells of claim 2, wherein disruption of the YDR155c gene is the result of deletion of all or part of the YDR155c gene.

4. The modified cells of claim 2, wherein disruption of the YDR155c gene is the result of deletion of a portion of genomic DNA comprising the YDR155c gene.

5. The modified cells of claim 2, wherein disruption of the YDR155c gene is the result of mutagenesis of the YDR155c gene.

6. The modified cells of any of claims 2-5, wherein disruption of the YDR155c gene is performed in combination with introducing a gene of interest at the genetic locus of the YDR155c gene.

7. The modified cells of any of claims 1-6, wherein the cells do not produce functional Cpr1 polypeptides.

8. The modified cells of any of claims 1-6, wherein the cells do not produce Cpr1 polypeptides.

9. The modified cells of any of claims 1-8, wherein the cells further comprise an exogenous gene encoding a carbohydrate processing enzyme.

10. The modified cells of any of claims 1-9, further comprising an alteration in the glycerol pathway and/or the acetyl-CoA pathway.

11. The modified cells of any of claims 1-10, further comprising an alternative pathway for making ethanol.

12. The modified cells of any of claims 1-11, further comprising a disruption of the YJL065 gene present in the parental cells.

13. The modified cells of any of claims 1-12, wherein the cells do not produce functional Dls1 polypeptides.

14. The modified cells of any of claims 1-13, wherein the cells are of a Saccharomyces spp.

15. The modified cells of any of claims 1-14, wherein the amount of ethanol produced by the modified yeast cells and the parental yeast cells is measured at 24 hours following inoculation of a hydrolyzed starch substrate comprising 34-35% dissolved solids and having a pH of 4.8-5.4.

16. A method for producing a modified yeast cell comprising:

introducing a genetic alteration into a parental yeast cell, which genetic alteration reduces or prevents the production of functional Cpr1 polypeptide compared to the parental cells, thereby producing modified cells that produces during fermentation an increased amount of ethanol compared to the parental cells under equivalent fermentation conditions.

17. The method of claim 16, wherein the genetic alteration comprises disrupting the YDR155c gene in the parental cells by genetic manipulation.

18. The method of claim 16 or 17, wherein the genetic alteration comprises deleting the YDR155c gene in the parental cells using genetic manipulation.

19. The method of any of claims 16-18, wherein disruption of the YDR155c gene is performed in combination with introducing a gene of interest at the genetic locus of the YDR155c gene.

20. The method of any of claims 16-19, wherein disruption of the YDR155c gene is performed in combination with making an alteration in the glycerol pathway and/or the acetyl-CoA pathway.

21. The method of any of claims 16-20, wherein disruption of the YDR155c gene is performed in combination with adding an alternative pathway for making ethanol.

22. The method of any of claims 16-21, wherein disruption of the YDR155c gene is performed in combination with disrupting the YJL065 gene present in the parental cells.

23. The method of any of claims 16-22, wherein disruption of the YDR155c gene is performed in combination with introducing an exogenous gene encoding a carbohydrate processing enzyme.

24. The method of any of claims 16-23, wherein the modified cell is from a Saccharomyces spp.

25. In some embodiments of the method of any of claims 16-24, the amount of ethanol produced by the modified yeast cells and the parental yeast cells is measured at 24 hours following inoculation of a hydrolyzed starch substrate comprising 34-35% dissolved solids and having a pH of 4.8-5.4.

26. Modified yeast cells produced by the method of any of claims 16-25.