ENGINEERED ELECTRODE FOR ELECTROBIOCATALYSIS AND PROCESS TO CONSTRUCT THE SAME
First Claim
1. A bio-electrode for electro-biocatalysis, wherein said bio-electrode comprises:
- electro-active bacteria layered and immobilized on the modified surface of the electrode with an immobilizing agent and conductive material.
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Abstract
The present disclosure provides a ready-to-use bio-electrode stable for long term storage and a process of constructing the same. The process for construction of bio-electrode for electro-biocatalysis comprising of: selection of an electro-active bacteria; enrichment of said electro-active bacteria in a nutrient rich medium; separation of said electro-active bacterial cells from said nutrient rich medium; selection of an electrode material; surface modification of said electrode material; layering the surface modified electrode material with conductive material; layering the surface modified electrode material with an electro-active bacterial cells along with biofilm inducing agents and stabilizing agents; conditioning the electro-active bacterial cells layered electrode; incubating the electrode obtained with an immobilizing agent along with conductive material; and conditioning the electrode with micronutrients to obtain a bio-electrode.
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Citations
17 Claims
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1. A bio-electrode for electro-biocatalysis, wherein said bio-electrode comprises:
electro-active bacteria layered and immobilized on the modified surface of the electrode with an immobilizing agent and conductive material. - View Dependent Claims (2, 3, 4, 5, 6, 7, 12)
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8. A process of construction of bio-electrode, wherein said process comprises of:
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(a) selection of an electro-active bacteria; (b) enrichment of said electro-active bacteria selected in step (a) in a nutrient rich medium; (c) separation of said electro-active bacterial cells obtained in step (b) from said nutrient rich medium; (d) selection of an electrode material; (e) surface modification of said electrode material selected in step (d); (f) layering the surface modified electrode material obtained in step (e) with conductive material and drying for 1-4 hours; (g) layering the surface modified electrode material obtained in step (f) with an electro-active bacterial cells obtained in step (c) along with biofilm inducing agents and stabilizing agents; (h) conditioning the electro-active bacterial cells layered electrode obtained in step (g); (i) incubating the electrode obtained in step (h) with an immobilizing agent along with conductive material for 2-3 hours; (j) conditioning the electrode obtained in step (i) with micronutrients to obtain a bio-electrode, wherein said bio-electrode is having shelf-life of at least 2-3 years. - View Dependent Claims (9, 10, 11, 13, 14, 15, 16, 17)
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Specification