CYCLIC PEPTIDE COMPOUND HAVING HIGH MEMBRANE PERMEABILITY, AND LIBRARY CONTAINING SAME

• US 20200131669A1
• Filed: 06/08/2018
• Published: 04/30/2020
• Est. Priority Date: 06/09/2017
• Status: Active Application

• Current Assignee: Chugai Seiyaku Kabushiki Kaisha (Roche Holding AG)
• Original Assignee: Chugai Seiyaku Kabushiki Kaisha (Roche Holding AG)
• Inventors: MURAOKA, Terushige, IIDA, Takeo, MATSUO, Atsushi, TAKEYAMA, Ryuichi, TANADA, Mikimasa, TANAKA, Shota, HAYASHI, Ryuji, FUJINO, Machiko, IIKURA, Hitoshi
• Application Number:
  US16/619,014
• Publication Number:
  US 20200131669A1
• Time in Patent Office: Days
• CPC Class Codes: C07K 1/13 Labelling of peptides
• CPC Class Codes: C07K 2/00 Peptides of undefined number of amino acids; Derivatives thereof
• CPC Class Codes: C07K 7/64 Cyclic peptides containing only normal peptide links
• CPC Class Codes: C12N 15/09 Recombinant DNA-technology
• CPC Class Codes: C12P 21/02 having a known sequence of two or more amino acids, e.g. glutathione
• CPC Class Codes: C40B 30/04 by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
• CPC Class Codes: C40B 40/04 Libraries containing only organic compounds
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: G01N 33/566 using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds

Abstract

The present inventors have found that when screening for cyclic peptide compounds that can specifically bind to a target molecule, the use of a library including cyclic peptide compounds having a long side chain in the cyclic portion can improve the hit rate for cyclic peptide compounds that can specifically bind to the target molecule. Meanwhile, the present inventors have found that tryptophan and tyrosine residues, which have conventionally been used in oral low molecular-weight pharmaceuticals and are amino acid residues having an indole skeleton or a hydroxyphenyl group, are not suitable for peptides intended to attain high membrane permeability.

Claims

1. A library of cyclic peptide compounds, wherein the library substantially consists of cyclic peptide compounds which do not have (1) an indole skeleton or (2) a substituted or unsubstituted hydroxyphenyl group in the side chains of the cyclic portion.

2. The library according to claim 1, wherein if the library comprises cyclic peptide compounds having an acidic side chain in the cyclic portion, the cyclic peptide compounds substantially consist of cyclic peptide compounds the acidic side chain of which has a pKa of 3.5 to 10.

3. The library according to claim 1 or 2, wherein if the library comprises cyclic peptide compounds having a basic side chain in the cyclic portion, the cyclic peptide compounds substantially consist of cyclic peptide compounds the basic side chain of which has a basic pKa of 4.0 to 10.

4. The library according to any one of claims 1 to 3, wherein the library comprises cyclic peptide compounds having a long side chain of 5.4 to 13 angstroms in length in the cyclic portion.

5. The library according to any one of claims 1 to 4, wherein the long side chain is a side chain comprising no amide bond or one amide bond in the side chain.

6. The library according to any one of claims 1 to 5, wherein the average of the number of amino acids constituting the cyclic portion of each cyclic peptide compound comprised in the library is 5 to 15.

7. The library according to any one of claims 1 to 6, wherein the average of the number of amino acids constituting the peptide moiety excluding the nucleic acid-linked portion of each cyclic peptide compound comprised in the library is 5 to 20.

8. The library according to any one of claims 1 to 7, wherein the average of the percentage of the number of N-substituted amino acids relative to the number of amino acids comprised in the peptide moiety excluding the nucleic acid-linked portion of each cyclic peptide compound comprised in the library is 30% or higher.

9. The library according to any one of claims 4 to 8, wherein the long side chain comprises R¹, wherein R¹ is:

(a) a phenyl group, or (b) a 4- to 6-membered heterocyclic group having 1 to 3 heteroatoms independently selected from the group consisting of an oxygen atom and a nitrogen atom in the ring,

wherein (a) and (b) optionally have at least one substituent independently selected from Group A below;

Group A;

oxo, a halogen atom, a C1-C4 alkyl group optionally substituted with a halogen atom, and a C1-C4 dialkylamino group (wherein one or two non-adjacent methylene groups in the C1-C4 alkyl group are optionally substituted with an oxygen atom);

or

(c) a group represented by the following general formula (II), (III), or (IV);

10. The library according to any one of claims 1 to 9, wherein the average of the number of aromatic rings comprised in the side chains of the cyclic portion of each cyclic peptide compound comprised in the library is 0 to 3.

11. A method of screening for a cyclic peptide compound that can specifically bind to a target molecule, the method comprising the steps of:

(i) contacting a cyclic peptide compound comprised in the library according to any one of claims 1 to 10 with the target molecule; and

(ii) selecting a cyclic peptide compound that can specifically bind to the target molecule.

14. A method of producing a cyclic peptide compound, the method comprising the steps of:

(i) contacting a cyclic peptide compound comprised in the library according to any one of claims 1 to 10 with a target molecule;

(ii) selecting a cyclic peptide compound capable of binding to the target molecule; and

(iii) producing a cyclic peptide compound based on the amino acid sequence of the cyclic peptide compound selected in (ii).

15. A cyclic peptide compound, wherein:

(1) the cyclic peptide compound does not have a methylthio group, a thiol group, an indole skeleton, or a substituted or unsubstituted hydroxyphenyl group in the side chains of the cyclic portion;

(2) if the cyclic peptide compound has an acidic side chain in the cyclic portion, the acidic side chain has a pKa of 3.5 to 10;

(3) if the cyclic peptide compound has a basic side chain in the cyclic portion, the basic side chain has a basic pKa of 4.0 to 10;

(4) the cyclic peptide compound has a long side chain having a side chain length of 6.0 to 13 angstroms in the cyclic portion; and

(5) the long side chain comprises R¹, wherein R¹ is as defined in claim 9.

12. A cell-free translation system for producing a peptide compound, wherein the cell-free translation system does not substantially comprise (a), (b), or (c) below:

(a) an amino acid having an indole skeleton in the side chain, or a nucleic acid encoding the amino acid;

(b) an amino acid having a fused-ring structure formed by two or more aromatic rings in the side chain, or a nucleic acid encoding the amino acid; and

(c) an amino acid having a substituted or unsubstituted hydroxyphenyl group in the side chain, or a nucleic acid encoding the amino acid.

13. A method of producing a library of peptide compounds, the method comprising at least one step selected from the group consisting of(a) and (b) below:

(a) preparing an amino acid pool substantially not comprising an amino acid which has an indole skeleton in the side chain, and synthesizing a peptide compound using a part or all of the amino acids comprised in the pool as constituent amino acids; and

(b) preparing a template pool substantially not comprising a nucleic acid encoding an amino acid which has an indole skeleton in the side chain, and synthesizing a peptide compound from the template pool.