METHOD FOR ASSAYING CATIONIC POLYMERS BY TIME-RESOLVED PHOTOLUMINESCENCE

• US 20200132602A1
• Filed: 02/21/2018
• Published: 04/30/2020
• Est. Priority Date: 03/27/2017
• Status: Active Grant

• Current Assignee: SPCM SA
• Original Assignee: SPCM SA
• Inventors: FAVERO, Cedrick, SOUZY, Renaud, BRAUN, Olivier, GUILLARD, Alexis
• Granted Patent: US 11,287,378 B2
• Time in Patent Office: Days
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 21/643 non-biological material
• CPC Class Codes: G01N 33/18 Water
• CPC Class Codes: G01N 33/2835 specific substances contained in the oil or fuel
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/582 with fluorescent label

Abstract

The present invention concerns a method for determining the concentration of cationic polymers present in a sample, according to the following steps: —bringing the cationic polymer or polymers present in the sample into contact with, and enabling the interaction thereof with, a developer solution comprising lanthanide (III) ions and at least one bonding agent, and —exciting the sample at an excitation wavelength λ_exc and detecting, by time resolved photoluminescence, a signal originating from the lanthanide (III) ions having interacted with the at least one bonding agent having previously interacted with the cationic polymer or polymers, at an emission wavelength λ_em, and —determining the cationic polymer concentration of the sample by using the signal detected at the emission wavelength λ_em, the sample originating from water originating from municipal or industrial water or sludge treatment processes.

Claims

1. A method for determining the concentration of cationic polymers present in a sample, according to the following steps:

bringing the cationic polymer(s) present in the sample into contact with, and enabling the interaction thereof with, a developer solution comprising lanthanide (III) ions and at least one bonding agent,

exciting the sample at an excitation wavelength λ

_exc and detecting, by time-resolved photoluminescence, a signal from the lanthanide (III) ions that have interacted with at least one bonding agent having previously interacted with the cationic polymer(s) at an emission wavelength Δ

_em, and

determining the cationic polymer concentration of the sample using the signal detected at the emission wavelength Δ

_em,

the sample originating from water obtained from water treatment processes or from municipal or industrial sludge.

2. The method according to claim 1, wherein the time-resolved photoluminescence is time-resolved fluorescence.

3. The method according to claim 1, wherein the sample contains at least one polymer resulting from the polymerization of at least one cationic monomer and, optionally, at least one non-ionic monomer (A) and/or at least one anionic or zwitterionic monomer.

4. The method according to claim 1, wherein the lanthanide ions are chosen from europium, terbium, samarium or dysprosium ions.

5. The method according to claim 4, wherein the lanthanide ions are europium or terbium ions.

6. The method according to claim 1, wherein an amount of lanthanide (III) ions between 1 ppm and 10,000 ppm is added to the sample by weight based on the weight of the sample before the sample comes into contact with the developer solution.

7. The method according to claim 1, wherein the excitation wavelength Δ

_exc is between 200 nm and 600 nm, and the emission signal wavelength Δ

_em is between 300 nm and 800 nm.

8. The method according to claim 1, wherein the bonding agent is an anionic agent comprising at least 2 anionic functions.

9. The method according to claim 1, further comprising a sample purification step prior to adding the developer solution comprising the lanthanide (III) ions.