DETECTION CARTRIDGE, DETECTION METHOD, AND DETECTION DEVICE

• US 20200132637A1
• Filed: 09/11/2019
• Published: 04/30/2020
• Est. Priority Date: 10/31/2018
• Status: Active Grant

• Current Assignee: Skyla Corporation (Lite-On Technology Corporation)
• Original Assignee: Skyla Corporation (Lite-On Technology Corporation)
• Inventors: Wei, Chia-Chun
• Granted Patent: US 11,366,083 B2
• Time in Patent Office: Days
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/0883 Serpentine channels
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: G01N 1/38 Diluting, dispersing or mixing samples
• CPC Class Codes: G01N 2001/383 collecting and diluting in a flow of liquid
• CPC Class Codes: G01N 29/02 Analysing fluids using acoustic emission techniques G01N29/14 ; constructional or flow details for analysing fluids G01N29/222; optoacoustic fluid cells G01N29/2425
• CPC Class Codes: G01N 29/222 Constructional or flow details for analysing fluids optoacoustic fluid cells G01N29/2425

Abstract

A detection cartridge, a detection method, and a detection device are provided. The detection cartridge includes a detection tank, a sample tank, N containers, and at least one first temporary tank. The sample tank is in communication with the detection tank. The N containers are in communication with the detection tank, wherein N is a positive integer greater than or equal to 2. The at least one first temporary tank is disposed on at least one of N flow paths between the N containers and the detection tank, wherein a quantity of the first temporary tanks on an nth flow path in the N flow paths is greater than or equal to that on an (n-1)th flow path, and n is a positive integer that is not less than 2 and is not more than N.

Claims

1. A detection cartridge, comprising:

a detection tank;

a sample tank in communication with the detection tank;

N containers in communication with the detection tank, wherein N is a positive integer greater than or equal to 2; and

at least one first temporary tank disposed on at least one of N flow paths between the N containers and the detection tank, wherein a quantity of the temporary tanks on an nth flow path in the N flow paths is greater than or equal to that on an (n-1)th flow path, and n is a positive integer that is not less than 2 and is not more than N.

2. The detection cartridge of claim 1, wherein the sample tank is not located on the N flow paths.

3. The detection cartridge of claim 1, wherein the at least one first temporary tank is located at a turning point of the N flow paths.

4. The detection cartridge of claim 1, further comprising a waste tank in communication with the detection tank.

5. The detection cartridge of claim 4, further comprising a centrifuge tank in communication between the sample tank and the detection tank.

6. The detection cartridge of claim 5, wherein the centrifuge tank and the at least one first temporary tank are respectively in communication with the waste tank.

7. The detection cartridge of claim 1, further comprising:

a second temporary tank in communication with the detection tank; and

a capture unit connected to an inner wall of the second temporary tank.

8. The detection cartridge of claim 1, further comprising a capture unit connected to an inner wall of the at least one first temporary tank.

9. The detection cartridge of claim 1, wherein some of the N flow paths have a shared path.

10. The detection cartridge of claim 9, wherein an (n-1)th container in the N containers is located on the shared path between a nth container and the detection tank.

11. A detection method, comprising:

providing a centrifuge device, wherein the centrifuge device comprises a driving unit, a platform connected to the driving unit, and a turntable disposed on the platform, wherein the platform has an axis of rotation;

mounting the detection cartridge of claim 1 on the turntable;

placing a sample into the sample tank;

placing N reagents into the N containers respectively;

introducing the sample into the detection tank;

introducing the N reagents into the detection tank sequentially via the N flow paths, wherein in the step of sequentially introducing the N reagents into the detection tank via the N flow paths, a rotating speed and a rotating direction of the platform are controlled to change a relative position of any position on the detection cartridge with respect to the axis of rotation, and a centrifugal force is applied to the sample and the N reagents; and

detecting a liquid in the detection tank.

12. The detection method of claim 11, wherein the detection cartridge has an angle of rotation with respect to a reference direction, and the angle of rotation is set according to a flow path in which a position of a liquid is to be changed in the N flow paths.

13. The detection method of claim 12, wherein the detection cartridge is located at the angle of rotation, and when the centrifugal force is applied to the sample and the N reagents, a position of the liquid in one part of the N flow paths is changed, and the liquid in the other part of the N flow paths remains at the same position.

14. The detection method of claim 11, wherein the detection cartridge further comprises a centrifuge tank in communication between the sample tank and the detection tank.

15. The detection method of claim 11, wherein the detection cartridge further comprises a waste tank in communication with the detection tank.

16. The detection method of claim 15, wherein the step of introducing the N reagents into the detection tank sequentially via the N flow paths comprises:

introducing a liquid in an (n-1)th flow path into the detection tank;

introducing the liquid in the detection tank into the waste tank; and

introducing a liquid in an nth flow path into the detection tank.

17. The detection method of claim 15, wherein the detection cartridge further comprises:

a second temporary tank in communication with the detection tank; and

a capture unit connected to an inner wall of the second temporary tank.

18. The detection method of claim 17, further comprising, before the liquid in the detection tank is introduced into the waste tank:

introducing the liquid in the detection tank into the second temporary tank; and

returning the liquid in the second temporary tank to the detection tank.

19. The detection method of claim 11, wherein the detection cartridge further comprises a capture unit, and the capture unit is connected to an inner wall of the at least one first temporary tank.

20. A detection device, comprising:

a centrifuge device, comprising;

a driving unit;

a platform connected to the driving unit; and

a turntable disposed on the platform; and

the detection cartridge of claim 1 mounted on the turntable.