Methods and Systems for Signal Amplification of Bioassays
First Claim
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1. A method for signal amplification comprising:
- adding to the analyte of interest a detection support comprising a solid support comprising a plurality of molecules of a binding agent that recognizes and binds to the analyte of interest; and
detecting at least some of the plurality of binding agent molecules on the detection support.
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Abstract
Disclosed herein are methods and systems for the detection of an analyte of interest by an amplified assay. The method and systems of the invention utilize the high specificity of biomolecules that have been coupled to a solid support to further amplify a signal produced by the interaction of a binding agent with the analyte of interest as a means to detect low levels of the analyte of interest present in a sample. In certain embodiments, for immunochemical (for example, ELISA or RIA) or immunofluorescence assays in which a protein to be identified is bound directly or through capture antibodies to a passivated surface, amplification of ˜10,000-fold can be achieved.
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Citations
30 Claims
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1. A method for signal amplification comprising:
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adding to the analyte of interest a detection support comprising a solid support comprising a plurality of molecules of a binding agent that recognizes and binds to the analyte of interest; and detecting at least some of the plurality of binding agent molecules on the detection support. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A kit for assaying an analyte of interest comprising:
- a detection support, the detection support comprising solid support comprising a plurality of molecules of a binding agent that can recognize and bind to the analyte of interest.
- View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
Specification