SRM/MRM Assays For Notch Pathway Proteins
First Claim
1. A method for measuring the level of protein in a biological sample of formalin fixed tissue, the method comprisingdetecting and/or quantifying by mass spectrometry an amount of one or more modified or unmodified fragment peptides derived from the protein in a protein digest prepared from said biological sample;
- and calculating the level of said protein in said sample;
wherein said protein is selected from the group consisting of DLL1, DLL3, JAG1, JAG2, Notch1, Notch 2, Notch 3, Notch 4, and DLL4 protein.
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Abstract
Methods are provided for detecting and quantifying the DLL1, DLL3, JAG1, JAG2, Notch1, Notch 2, Notch 3, Notch 4, and/or DLL4 proteins in biological samples, such as a formalin fixed paraffin embedded tissue samples, using mass spectrometry. Additionally, methods are provided for treating cancer based upon the level of the DLL1, DLL3, JAG1, JAG2, Notch1, Notch 2, Notch 3, Notch 4, and/or DLL4 proteins in the biological samples.
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Citations
26 Claims
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1. A method for measuring the level of protein in a biological sample of formalin fixed tissue, the method comprising
detecting and/or quantifying by mass spectrometry an amount of one or more modified or unmodified fragment peptides derived from the protein in a protein digest prepared from said biological sample; - and calculating the level of said protein in said sample;
wherein said protein is selected from the group consisting of DLL1, DLL3, JAG1, JAG2, Notch1, Notch 2, Notch 3, Notch 4, and DLL4 protein. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
- and calculating the level of said protein in said sample;
Specification