A PROTEOLIPOSOME-BASED ZNT8 SELF-ANTIGEN FOR TYPE 1 DIABETES DIAGNOSIS
First Claim
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1. A method of detecting ZNT8 antibodies in serum comprising:
- providing a collection of proteoliposomes having an interior and an exterior wherein some proteoliposomes have protein sequences of a transmembrane domain (TMD) of a ZnT8 on its exterior and other proteoliposomes comprise protein sequences of a cytosolic domain (CTD) of a ZnT8 protein exposed on its exterior;
adding serum comprising antibodies targeting the protein sequences of TMD and/or CTD forming proteoliposomes bound to the antibodies;
adding a labelled captured autoantibody that binds to the antibodies bound to the proteoliposomes; and
measuring the amount of antibodies targeting the protein sequences of TMD and/or CTD bound to the proteoliposomes.
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Abstract
Methods of detecting ZNT8 antibodies in serum are described. The methods include proteoliposomes comprising a transmembrane domain (TMD) and a cytosolic domain (CTD) of ZnT8 proteins exposed on the exterior of the proteoliposome; serum comprising antibodies targeting the ZnT8 proteins; and labelled captured autoantibodies that bind to ZnT8 antibodies.
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Citations
23 Claims
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1. A method of detecting ZNT8 antibodies in serum comprising:
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providing a collection of proteoliposomes having an interior and an exterior wherein some proteoliposomes have protein sequences of a transmembrane domain (TMD) of a ZnT8 on its exterior and other proteoliposomes comprise protein sequences of a cytosolic domain (CTD) of a ZnT8 protein exposed on its exterior; adding serum comprising antibodies targeting the protein sequences of TMD and/or CTD forming proteoliposomes bound to the antibodies; adding a labelled captured autoantibody that binds to the antibodies bound to the proteoliposomes; and measuring the amount of antibodies targeting the protein sequences of TMD and/or CTD bound to the proteoliposomes. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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23. A method of detecting ZNT8 antibodies in serum comprising:
forming a mixture by combining proteoliposomes having an interior and an exterior comprising protein sequences of a transmembrane domain (TMD) and a cytosolic domain (CTD) of a ZnT8 proteins exposed on the exterior of the proteoliposomes;
serum comprising antibodies targeting the protein sequences;
a labelled captured autoantibody that binds to the antibodies targeting the protein sequences; and
measuring the amount of antibodies bound to the protein sequences.
Specification