Process of producing insulin crystals of substantially uniform size and compositions thereof

• US 2,799,622 A
• Filed: 11/12/1954
• Issued: 07/16/1957
• Est. Priority Date: 11/14/1953
• Status: Expired due to Term

• Current Assignee: Jphirgen Schlichtkrull, Merete Nphiring Inger
• Original Assignee: Jphirgen Schlichtkrull, Merete Nphiring Inger
• Inventors: JPHIRGEN SCHLICHTKRULL, MERETE NPHIRING INGER
• Application Number:
  US03/468,541
• Time in Patent Office: 977 Days
• US Class Current: 514/6.4
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/625 Extraction from natural sources

Claims

1. IN A PROCESS OF PRODUCING INSULIN CRYSTALS OF PREDETERMINED SUBSTANTIALLY UNIFORMED SIZE FROM AN INSULINCONTAINING AQUEOUS MEDIUM HAVING A PH-VALUE BETWEEN 5 AND 7 IN THE PRESENCE OF A CRYSTALLIZATION PROMOTING METAL, THE STEP WHICH COMPRISES SEEDING SAID MEDIUM WITH INSULIN CRYSTALS OF SUBSTANTIALLY UNIFORM SIZE IN AN AMOUNT CORRESPONDING TO THE EQUATION:

4. AN INJECTABLE INSULIN PREPARATION COMPRISING AN AQUEOUS MEDIUM MEDIUM HAVING INSULIN CRYSTALS SUSPENDED THEREIN, SAID MEDIUM AND SAID CRYSTALS CONTAINING IN NON-TOXIC AMOUNTS AT LEAST ONE METAL SELECTED FROM THE GROUP CONSISTING OF ZINC, COBALT, NICKEL, CADMIUM, COPPER, MANGANESE AND IRON, THE METAL CONTENT OF THE SUSPENDED CRYSTALS BEING AT LEAST 0.25 MILLIEQUIVALENT PER GRAM OF CRYSTALS WHEN SAID PREPARATION HAS A PH OF ABOUT 7, AND THE MAJOR PROPORTION BY WEIGHT OF SAID CRYSTALS HAVING A SUBSTANTIALLY UNIFORM SIZE, SAID CRYSTALS BEING FORMED BY SEEDING AN INSULIN-CONTAINING AQUEOUS MEDIUM HAVING A PH VALUE BETWEEN 5 AND 7 AND CONTAINING A CRYSTAILIZATIONPROMOTING METAL WITH INSULIN CRYSTALS KOF SUBSTANTIALLY UNIFORM SIZE IN AN AMOUNT CORRESPONDING TO THE EQUATION: