Process for crystallization of insulin using freeze dried insulin as seeding material

• US 2,819,999 A
• Filed: 11/12/1954
• Issued: 01/14/1958
• Est. Priority Date: 11/13/1953
• Status: Expired due to Term

• Current Assignee: Jorgen Schlichtkrull, Merete Noring Inger
• Original Assignee: Jorgen Schlichtkrull, Merete Noring Inger
• Inventors: JORGEN SCHLICHTKRULL, MERETE NORING INGER
• Application Number:
  US03/468,542
• Time in Patent Office: 1,159 Days
• US Class Current: 514/6.4
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/625 Extraction from natural sources

Claims

1. IN A PROCESS OF PRODUCING INSULIN CRYSTALS FROM AN AQUEOUS INSULIN-CONTAINING MEDIUM BY ADJUSTING THE PH OF SAID MEDIUM TO A VALUE BETWEEN 5 AND 7, SAID MEDIUM CONTAINING AT LEAST ONE METAL SELECTED FROM THE GROUP CONSISTING OF ZINC, COBALT, NICKEL, CADMIUM, COPPER, MANGANESE AND IRON IN BIVALENT FORM, THE STEP WHICH COMPRISES ADDING FREEZE-DRIED INSULIN TO SAID MEDIUM BEFORE THE CRYSTALS BEGIN TO BE FORMED, WHEREBY THE INSULIN CRYSTALS APPEAR IN THE FORM OF INDIVIDUAL CRYSTAL BODIES OF SUBSTANTIALLY THE SAME SIZE.