Preparation of insulin containing 2.75 to 8 percent zinc content

• US 3,102,077 A
• Filed: 10/27/1960
• Issued: 08/27/1963
• Est. Priority Date: 08/19/1953
• Status: Expired due to Term

• Current Assignee: Marinus Christensen Henry
• Original Assignee: Marinus Christensen Henry
• Inventors: MARINUS CHRISTENSEN HENRY
• Application Number:
  US04/065,408
• Time in Patent Office: 1,034 Days
• US Class Current: 530/304
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/62 Insulins

Claims

1. A PROCESS FOR THE PRODUCTION OF CRYSTALLINE ZINCINSULIN COMPOUNDS HAVING A CONENT OF MONOVALENT ANION SELECTED FROM THE GROUP CONSISTING OF CHLORIDE, BROMIDE AND ACETATE CORRESPONDING STOICHIOMETRICALLY TO TWO GRAM ATOMS OF HEAVY METAL LESS THAN THE TOTAL AMOUNT OF GRAM ATOMS OF SAID HEAVY METAL PRESENT IN SAID METAL-INSULIN COMPOUND PER MOLE THEREOF, WHICH PROCESS COMPRISES REACTING INSULIN WITH THE SALT OF SAID MONOVALENT ANION AND A HEAVY METAL SELECTED FROM THE GROUP CONSISTING OF ZINC, CALCIUM, MANGANESE, COBALT, MAGNESIUM, IRON, NICKEL AND CADMIUM IN A SOLVENT FO SAID SALT, SID SOLVENT BEING CHEMICALLY INERT TO INSULIN AND TO THE FORMED METAL-INSULIN COMPOUND AND BEING SELECTED FROM THE GROUP CONSISTING OF ANHYDROUS LOWER ALKYL ALCOHOLS, AQUEOUS MIXTURES OF LOWER ALKYL ALCOHOLS, ANHYDROUS LOWER ARALKYL ALCOHOLS, UNSUBSTITUTED AND LOWER ALKYL-SUBSTITUTED PHENOLS, ANHYDROUS PYRIDINES, AQUEOUS PYRIDINE MIXTURES ALONE AND IN MIXTURE WITH AMMONIA-AMMONIUM CHLORIDE, LOWER MONO- AND DI-BASIC FATTY ACIDS AND MIXTURES OF SAID FATTY ACIDS WITH WATER ALONE AND TOGETHER WITH AN ADMIXTURE OF AMMONIA-AMMONIUM CHLORIDE, THE CONTENT OF WATER IN SAID MIXTURES WITH LOWER ALKYL ALCOHOLS, WITH PYRIDINES AND WITH SAID FATTY ACIDS, RESPECTIVELY, BEING LESS THAN 85% BY VOLUME.