Production of carotenoid pigments

• US 3,108,402 A
• Filed: 03/16/1961
• Issued: 10/29/1963
• Est. Priority Date: 03/16/1961
• Status: Expired due to Term

• Current Assignee: Henry R. Kathrein
• Original Assignee: Henry R. Kathrein
• Inventors: Kathrein, Henry R.
• Application Number:
  US04/096,095
• Time in Patent Office: 957 Days
• US Class Current: 435/67
• CPC Class Codes: B62D 29/00 Superstructures, understructures, or sub-units thereof, characterised by the material thereof B62D33/044, B62D33/048 take precedence
• CPC Class Codes: C12P 23/00 Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes containing heterorings C12P17/00
• CPC Class Codes: Y10S 435/813 Continuous fermentation
• CPC Class Codes: Y10S 435/946 using algae

Claims

1. A PROCESS FOR THEPRODUCTION OF CAROTENOIDS WHICH COMPRISES CULIVATING, AT A TEMPERATURE OF FROM 10 TO 40% C. AND A PH OF FROM 5.4 TO 9 UNDER HETERTROPHIC CONDITIONS, A GREEN ALGA OF THE DIVISION CHLOROPHYTA AND OF THE GENUS SELECTED FROM THE GROUP CONSISTING OF CHLORELLA, CHLOROCOCCUM, SPONGIOCHLORIS, SPONGIOCOCCUM, AND COCCYMYXA IN AN AQUEOUS ORGANIC NUTRIENT MEDIUM COMPRISING A PROTEINACEOUS MATERIAL, FROM 0.05 TO 4 PERCENT UREA AND INITIALLY UP TO ABOUT 9 PERCENT BY WEIGHT (DRY BASIS) CARBOHYDRATE, SAID PROCESS BEING FURTHER CHARACTERIZED IN THE FURTHER ADDITION OF CARBOHYDRATE TO SAID NUTRIENT MEDIUM DURING THE CULTIVATION TO MAINTAIN THE CONCENTRATION THEREOF AT FROM ABOUT 0.5 TO ABOUT 6.0 PERCENT (DRY BASIS) AND WHEREIN THE AGGREGATE CARBOHYDRATE USED IN THE NUTRIENT DURING THE CULTIVATION IF FROM ABOUT 4 TO 30 PERCENT BY WEIGHT (DRY BASIS).