Method of producing a proteolytic enzyme by use of black aspergillus type molds
First Claim
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1. A PROCESS FOR MAKING ACID-PROTEASE HAVING AN OPTIMUM PH OF ABOUT 2.7 WHICH COMPRISES CULTIVATING ASPERGILLUS USAMII IN A MEDIUM CONTAINING INORGANIC NITROGEN SOURCE IN AN AMOUNT SUCH THAT THE C/N RATIO OF THE MEDIUM IS BELOW 3.2 AT ABOUT 30*C. FOR AT LEAST 60 HOURS.
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1 Claim
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1. A PROCESS FOR MAKING ACID-PROTEASE HAVING AN OPTIMUM PH OF ABOUT 2.7 WHICH COMPRISES CULTIVATING ASPERGILLUS USAMII IN A MEDIUM CONTAINING INORGANIC NITROGEN SOURCE IN AN AMOUNT SUCH THAT THE C/N RATIO OF THE MEDIUM IS BELOW 3.2 AT ABOUT 30*C. FOR AT LEAST 60 HOURS.
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Current AssigneeEiji Ichishima, Fumihiko Yoshida
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Original AssigneeEiji Ichishima, Fumihiko Yoshida
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InventorsFUMIHIKO YOSHIDA, EIJI ICHISHIMA
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Application NumberUS04/135,416Time in Patent Office1,112 DaysField of SearchUS Class Current435/225CPC Class CodesC12N 9/62 from AspergillusY10S 435/913 Aspergillus
