Concentration and purification of interferons, viral inhibiting substances (vis), viral inhibiting factors (vif), or viral inhibitory material

• US 3,256,152 A
• Filed: 06/14/1965
• Issued: 06/14/1966
• Est. Priority Date: 06/14/1965
• Status: Expired due to Term

• Current Assignee: Lampson, George P.
• Original Assignee: Lampson, George P.
• Inventors: Lampson, George P.
• Application Number:
  US04/463,907
• Time in Patent Office: 365 Days
• US Class Current: 435/70.5
• CPC Class Codes: C07K 14/555 Interferons [IFN]
• CPC Class Codes: Y10S 435/811 Interferon

Claims

1. A PROCESS FR CONCENTRATING INTERFERONS FROM A FLUID MEDIUM INTO WHICH INTERFERON HAS BEEN ELABORATED BY CELLS IN CONTACT WITH INTERFERON INDUCING AGENTS COMPRISING ADDING TO SAID FLUID MEDIUM MAINTAINED AT A TEMPERATURE BETWEEN 0 TO 30*C. A PROTEIN PRECIPITATING AGENT SELECTED FROM THE GROUP CONSISTING OF PERCHLORIC ACID, TRICHLORACETIC ACID, SULFOSALICYLIC ACID AND TETRAMETAPHOSPHATE TO A FINAL CONCENTRATION OF 1-3% OF SAID INTERFERON-CONTAINING FLUID, ADJUSTING THE SUPERNATE TO PH 6-8 AND THEN ADDING A HEAVY METAL SALT SELECTED FROM THE GROUP CONSISTING OF COBALT, ZINC, CALCIUM, MERCUTY, STRONTIUM AND BARIUM SALTS OF ACETIC ACID, GLYCINE, LACTIC ACID, PROPIONIC ACID AND HYDROCHLORIC ACID TO COPRECIPITATE THE INTERFERON.