Extraction and purification of chlorophyll

• US 3,258,467 A
• Filed: 04/17/1963
• Issued: 06/28/1966
• Est. Priority Date: 04/17/1963
• Status: Expired due to Term

• Current Assignee: Anderson Alexander F H, Melvin Calvin
• Original Assignee: Anderson Alexander F H, Melvin Calvin
• Inventors: ANDERSON ALEXANDER F H, MELVIN CALVIN
• Application Number:
  US04/273,797
• Time in Patent Office: 1,168 Days
• US Class Current: 540/145
• CPC Class Codes: C07D 487/22 in which the condensed system contains four or more hetero rings
• CPC Class Codes: G01N 2030/062 extracting sample from raw material
• CPC Class Codes: G01N 30/06 Preparation
• CPC Class Codes: G01N 33/48 Biological material, e.g. blood, urine G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence; Haemocytometers counting blood corpuscules distributed over a surface by scanning the surface G06M11/02

Claims

1. IN A METHOD OF OBTAINING PURE CHLOROPHYLL VALUES FROM AN UNREFINED EXTRACTANT OBTAINED BY SOLVENT EXTRACTION OF GREEN PLANTS, THE STEPS COMPRISING, ADSORBING THE EXTRACTANT ON POWDERED POLYOLEFIN, SAID POLYOLEFIN BEING SELECTED FROM THE GROUP CONSISTING OF POLYETHYLENE AND POLYPROPYLENE, CHROMATOGRAPHICALLY ELUTING XANTHOPHYLL FROM SAID POLYOLEFIN, FURTHER ELUTING SAID POLYOLEFIN TO COLLECT CHLOROPHYLL VALUES, READSORBING SAID CHLOROPHYLL VALUES ON SUCROSE, AND SEQUENTIALLY CHROMATOGRAPHICALLY ELUTING PURIFIED CHLOROPHYLL VALUES FROM SAID SURCOSE.