Determination of oxidizing and reducing substances

• US 3,282,649 A
• Filed: 05/04/1962
• Issued: 11/01/1966
• Est. Priority Date: 05/04/1962
• Status: Expired due to Term

• Current Assignee: Donald L. Bittner
• Original Assignee: Donald L. Bittner
• Inventors: Bittner, Donald L.
• Application Number:
  US04/192,564
• Time in Patent Office: 1,642 Days
• US Class Current: 436/86
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/144444 Glucose
• CPC Class Codes: Y10T 436/148888 Uric acid

Claims

1. A METHOD FOR THE COLORIMETRIC QUANTITATIVE ANALYSIS OF REDUCING AND OXIDIZING SUBSTANCES CAPABLE OF REACTING WITH COPPER TO CHANGE THE VALENCE STATE OF THE COPPER COMPRISING:

(A) CONTACTING AT LEAST ONE OF SAID SUBSTANCES IN LIQUID PHASE WITH AN INDICATOR CONSISTING ESSENTIALLY OF A PREFORMED CHELATE OF SUBSTANTIALLY COMPLETELY BOUND COPPER WITH A MEMBER OF THE GROUP SELECTED FROM 2,2'"'"''"'"'-BIQUINOLINES AND 2,9-SUBSTITUTED-1,10-PHENANTHROLINES UNDER VALENCE CHANGING CONDITIONS FOR SAID COPPER;

(B) AND THEN MEASURING THE AMOUNT OF COPPER VALENCE CHANGE BY REFERENCE TO THE LIGHT TRANSMITTANCE OF SAID LIQUID PHASE, AND DETERMINING THE AMOUNT OF SUBSTANCE BEING ANALYZED BY COMPARING THE LIGHT TRANSMITTANCE TO A STANDARD.