PROCESS FOR PREPARING LYOPHILIZED HUMAN BLOOD PROTEINS SUCH AS GAMMA GLOBULIN IN THE PRESENCE OF A NONIONIC SURFACTANT

• US 3,607,858 A
• Filed: 03/31/1970
• Issued: 09/21/1971
• Est. Priority Date: 03/31/1970
• Status: Expired due to Term

• Current Assignee: Francis Fremonte Buck, Merle Vernon Querry
• Original Assignee: Francis Fremonte Buck, Merle Vernon Querry
• Inventors: Querry, Merle Vernon, Buck, Francis Fremonte
• Primary Examiner(s): Schain, Howard E.
• Application Number:
  US05/024,364
• Time in Patent Office: 539 Days
• Field of Search: 260/112B 424/101,177 34/5
• US Class Current: 424/130.1
• CPC Class Codes: C07K 16/06 from serum
• CPC Class Codes: Y10S 530/831 Cohn fractions

Abstract

Human blood proteins, such as alpha globulin, beta globulin, albumin, and particularly gamma globulin, are prepared in dry lyophilized form by freeze drying an aqueous solution in the presence of small amounts of nonionic surface active agents, the initial freezing being very rapid, from 1 to 3 minutes, preferably by freezing in small containers, such as vials, immersed in an alcohol Dry Ice bath.

Claims

2. A process according to claim 1 in which the protein is immune serum globulin.

3. A process according to claim 2 in which the rapid freezing is effected by immersing a small container containing the solution of the immune serum globulin in a bath of ethanol and Dry Ice.

4. A process according to claim 1 in which the rapid freezing is effected by immersing a small container containing the solution of the immune serum globulin in a bath of ethanol and Dry Ice.