METHOD OF AND APPARATUS FOR ELECTROPHORETIC SEPARATION IN A GEL COLUMN

• US 3,616,454 A
• Filed: 03/13/1969
• Issued: 10/26/1971
• Est. Priority Date: 03/13/1969
• Status: Expired due to Term

• Current Assignee: Isaac Schenkein, Milton Levy
• Original Assignee: Isaac Schenkein, Milton Levy
• Inventors: Levy, Milton, Schenkein, Isaac
• Primary Examiner(s): Edmundson, F. C.
• Application Number:
  US04/806,931
• Time in Patent Office: 957 Days
• Field of Search: 204/18G,299,18R,301
• US Class Current: 204/615
• CPC Class Codes: G01N 27/44756 Apparatus specially adapted therefor

Abstract

Method of and apparatus for the preparative gel electrophoresis of a specimen wherein the specimen is placed at the upper end of a polyacrylamide gel column of an electrophoresis gel whose lower end terminates at a receptacle containing an elution solution, an electrical potential of a magnitude sufficient to effect migration of components of the specimen to the lower end of said column is applied thereacross, and the solution is periodically drained from the receptacle and fresh elution buffer in corresponding quantities is introduced while the electrophoresis current is cut off. The column is supported on a layer of openpore high-wet-strength paper.

Claims

2. The improvement defined in claim 1 wherein said control means includes mechanism for feeding a corresponding quantity of fresh elution solution into said receptacle substantially in the cadence with the draining of said quantities of said elution solution therefrom, and means for terminating the application of said electrical potential during draining of said receptacle and the feeding of said fresh elution solution thereto.

3. The improvement defined in claim 1 wherein the means for applying said electrophoresis electrical potential across said gel column includes a further gel column having its end contacting said elution solution, a first electrode at said one end of the first-mentioned gel column in electrical connection therewith, a second electrode in electrical connection with said further gel column and a source of high-voltage direct current connected across said electrodes.

4. The improvement defined in claim 1, wherein said receptacle is provided with a downwardly extending discharge passage, said control means further comprising an electromagnetic valve along said passage triggerable to permit the gravity drain of said elution solution from said receptacle.

5. The improvement defined in claim 1, further comprising a fraction collector having a plurality of individual vessels successively alignable with said receptacle for collecting respective quantities of the elution solution drained from said receptacle.

6. The improvement defined in claim 1, further comprising a layer of open-pore high-wet-strength paper spanning the mouth of said gel column at said other end for supporting said gel column.