GLUCOSE DETERMINATION METHOD

• US 3,623,960 A
• Filed: 04/07/1970
• Issued: 11/30/1971
• Est. Priority Date: 04/07/1970
• Status: Expired due to Term

• Current Assignee: David L. Williams
• Original Assignee: David L. Williams
• Inventors: Williams, David L.
• Primary Examiner(s): Tung, T.
• Application Number:
  US05/026,362
• Time in Patent Office: 602 Days
• Field of Search: 204/1T,195
• US Class Current: 205/778
• CPC Class Codes: C12Q 1/006 for glucose

Abstract

An electrochemical apparatus especially applicable for clinical use in determining glucose concentrations in solutions thereof comprising (1) a sample reservoir, (2) a glucose oxidase member consisting essentially of a well closed at the bottom by a dialysis membrane and containing an anode, glucose oxidase and a buffer solution, (3) a reference member consisting essentially of a well closed at the bottom by a dialysis membrane and containing an anode and buffer solution, (4) a cathode, (5) means for positioning the members and the cathode in the sample reservoir, (6) means for applying a voltage between the cathode and the anodes, and (7) means for measuring the electrical currents produced. The process for using the apparatus comprises adding a buffered glucose solution to the sample reservoir, immersing the cathode and the members into the solution, adding a quinone either to the members or to the glucose solution, applying a voltage between the cathode and the anodes and measuring the currents produced.

Claims

2. A process as in claim 1 wherein the quinone is benzoquinone.

3. A process as in claim 1 wherein the dialysis membrane is cellulose.

4. A process as in claim 1 wherein the glucose solution is whole blood.