WATER-SOLUBLE POLYMER-ENZYME PRODUCTS
First Claim
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2. Product of claim 1, wherein the polymer is formed by polymerization of polymerizable acids or anhydrides, or by copolymerizing a polymerizable acid or anhydride with another copolymerizable monomer.
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Abstract
Polymer-enzyme products wherein the enzyme is covalently bound to the polymer chain, the polymer-enzyme product being water soluble. The products have wide applicability as stable, longacting, enzymatic materials, having same general type of activity as parent enzyme, but with different pH optimum activity and range of applicability, and are stable, substantially colorless and odorless, long acting, and remarkably less subject to autogenous deterioration or destruction by other enzymes.
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Citations
19 Claims
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2. Product of claim 1, wherein the polymer is formed by polymerization of polymerizable acids or anhydrides, or by copolymerizing a polymerizable acid or anhydride with another copolymerizable monomer.
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3. Product of claim 1, wherein the polymer is an EMA-type polymer.
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4. Product of claim 3, wherein the polymer is EMA.
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5. Product of claim 1, which is water-soluble EMA-lipase.
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6. Product of claim 1, which is water-soluble EMA-cellulase.
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7. Product of claim 1, which is water-soluble EMA-asparaginase.
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8. Product of claim 1, which is water-soluble EMA-alkaline protease.
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9. Product of claim 1, which is water-soluble EMA-neutral protease.
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10. Product of claim 1, which is water soluble EMA-amylase.
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11. Product of claim 1, wherein a plurality of different enzymes of microbiological origin are present in the water-soluble polymer-enzyme product.
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12. Product of claim 1, which is a water-soluble EMA-alkaline protease, neutral protease product.
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13. Product of claim 1, which is a water-soluble EMA-alkaline protease, neutral protease and amylase product.
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14. Product of claim 1, which is a water-soluble EMA-alkaline protease, neutral protease and lipase product.
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15. Product of claim 12, wherein the ratio of alkaline protease activity to neutral protease activity is about 0.25 to about 1.2 to 1.
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16. Product of claim 1, which is water-soluble EMA-dextranase.
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17. Product of claim 1, which is a water-soluble EMA-dextranase, neutral protease product.
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18. Method of producing a water-soluble enzymatically active polymer-enzyme product wherein the enzyme is bound covalently through groups which are nonessential for enzymatic activity to (a) a water-soluble polymer comprising chains of carboxylic acid or carboxylic acid anhydride units, or (b) a water-soluble polymer comprising units of carboxylic acid or carboxylic acid anhydride groups separated by carbon chains of at least one and not more than four carbon atoms, said carbon chains being part of a unit which contains a maximum of 18 carbon atoms, which comprises the step of reacting the polymer and an enzyme under substantially noncross-linking conditions and according to conditions which do not operate to destroy enzymatic activity to produce a desired enzymatically active water-soluble polymer-enzyme product, wherein the polymer and enzyme are reacted together in dilute aqueous solution or suspension or with the enzyme in sufficient concentration to favor production of a noncrosslinked polymer-enzyme product, and wherein the polymer is selected from the group consisting of an ethylene/maleic anhydride copolymer, styrene/maleic anhydride copolymer, vinyl methyl ether/maleic anhydride copolymer, vinyl acetate/maleic anhydride copolymer, divinyl ether/maleic anhydride cyclocopolymer, polymaleic anhydride, polyacrylic anhydride, and cationic derivatives thereof and wherein the enzyme moiety comprises at least one enzyme of microbial origin selected from the group consisting of neutral protease, acid protease, alkaline protease, lipase, cellulase, dextranase, amylase and asparaginase.
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19. Process of claim 18 wherein the polymer is reacted with a plurality of different enzymes of microbiological origin to produce a water-soluble polymer-enzyme product having a plurality of Different enzymes of microbiological origin bound therein.
Specification