NUTRITIVE PROTEIN FROM CELLULOSE

• US 3,627,095 A
• Filed: 08/05/1969
• Issued: 12/14/1971
• Est. Priority Date: 08/05/1969
• Status: Expired due to Term

• Current Assignee: Clayton D. Callihan, Vadake R. Srinivasan
• Original Assignee: Clayton D. Callihan, Vadake R. Srinivasan
• Inventors: Srinivasan, Vadake R., Callihan, Clayton D.
• Primary Examiner(s): Monacell, A. Louis
• Assistant Examiner(s): Nath, Gary M.
• Application Number:
  US04/847,719
• Time in Patent Office: 861 Days
• Field of Search: 195/1,8,9,33,111 99/2,9 71/9,10,11-70 200/212
• US Class Current: 435/71.2
• CPC Class Codes: A23K 10/12 by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
• CPC Class Codes: C12N 1/22 Processes using, or culture media containing, cellulose or hydrolysates thereof
• CPC Class Codes: Y10S 435/829 Alcaligenes

Abstract

Comestible, digestible protein is produced from cellulose by the combined cultivation of a cellulase-elaborating micro-organism and ALCALIGENES FAECALIS on delignified cellulose. The resultant protein is high in nutritive value and is suitable for use as an animal feedstuff.

Claims

2. A method according to claim 1 wherein the cellulose is delignified by contact with alkali followed by oxidation.

3. A method according to claim 1 wherein insoluble residual cellulose is separated by filtration subsequent to cultivation.

4. A method according to claim 3 wherein the cellulase-elaborating micro-organism and alcoligenes faecalis cells produced are harvested after the residual cellulose is separated.

5. A method according to claim 1 wherein the cellulase-elaborating micro-organism is of the genus Cellulomonas.

6. A method for the preparation of nutritive protein from cellulose which comprises cultivating Cellulomonas species ATCC No. 21399and Alcaligenes faecalis strain ATCC No. 21400on delignified cellulose under submerged aerobic conditions in an aqueous nutrient medium at a pH about 7 and a temperature about 32* C.