ELECTRODE SENSITIVE TO CHOLINE AND ITS ESTERS AND METHODS USING SAID ELECTRODE

• US 3,632,483 A
• Filed: 06/15/1970
• Issued: 01/04/1972
• Est. Priority Date: 06/15/1970
• Status: Expired due to Term

• Current Assignee: CIBA Vision Corporation (Bayer AG)
• Original Assignee: George Baum
• Inventors: Baum, George
• Primary Examiner(s): Kaplan, G. L.
• Application Number:
  US05/046,315
• Time in Patent Office: 568 Days
• Field of Search: 204/195L,1T 324/29 195/103.5
• US Class Current: 205/777.5
• CPC Class Codes: G01N 27/3335 the membrane containing at least one organic component G01N27/3271 takes precedence; aspects concerning the enzyme reagent in enzyme electrodes C12Q1/001
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

An electrode for measuring the concentration of cations of choline and its esters in an aqueous solution wherein the sensing portion is a liquid organic phase containing a substituted tetraphenylborate dissolved in a suitable organic solvent.

Claims

2. The electrode of claim 1, wherein said material is dissolved in a nitroaromatic solvent.

3. The electrode of claim 2, wherein said solvent has the formula wherein R is a member selected from the group consisting of hydrogen, alkyl containing 1-14 carbon atoms, lower alkoxy and alkylcarboxy, and R'"'"''"'"' is a member selected from the group consisting of hydrogen and methyl.

4. The electrode of claim 3, wherein said ion exchange material is choline tetrakis(p-chlorophenyl)borate.

5. The electrode of claim 3, wherein said ion exchange material is acetylcholine tetrakis(p-chlorophenyl)borate.

6. The electrode of claim 3 wherein said solvent is 1,2-dimethyl-4-nitrobenzene.

7. The electrode of claim 2 wherein said means includes a container for said liquid organic phase having an opening at a portion and an organophilic-hydrophobic porous membrane disposed in covering relationship across said opening.

8. The electrode of claim 7, wherein said membrane consists of a ceramic material coated wiTh a treating agent to impart the organophilic-hydrophobic property.

9. The electrode of claim 7 wherein a dialysis membrane is positioned in front of and across said porous membrane.

10. The electrode of claim 9 wherein said dialysis membrane consists essentially of porous cellophane.

11. A method of measuring the concentration of choline and its esters in an aqueous solution comprising the steps of a. providing a liquid organic ion exchange electrode wherein the liquid organic phase contains an ion exchange material having the formula ((CH3)3NCH2CH2OR) (B(C6H4X)4) wherein X is chlorine or phenoxy, R is hydrogen or and R'"'"''"'"' is lower alkyl or phenyl, b. subjecting the aqueous solution to said liquid organic ion exchange electrode and a reference electrode, and c. measuring the potential developed between the electrodes.

12. The method of claim 11 wherein said aqueous solution contains acetylcholine.

13. A method of determining the activity of an esterase in an aqueous solution containing acetylcholine and the esterase comprising the steps of a. providing a liquid organic ion exchange electrode wherein the liquid organic phase consists essentially of a nitroaromatic solvent and 0.5-10 percent by weight of an ion exchange material having the formula ((CH3)3NCH2CH2OR) (B(C6H4X)4) wherein X is chlorine or phenoxy, R is hydrogen or and R'"'"''"'"' is lower alkyl or phenyl, b. subjecting the aqueous solution to said liquid organic ion exchange electrode and a reference electrode, c. measuring the potential developed between the electrodes, and d. calculating the rate of loss of acetylcholine by enzymatic hydrolysis.

14. The method of claim 13, wherein said esterase is cholinesterase.

15. The method of claim 13, wherein said esterase is acetylcholinesterase.