ADAPTER FOR A MACROMOLECULE SEPARATION DEVICE

• US 3,640,813 A
• Filed: 06/09/1969
• Issued: 02/08/1972
• Est. Priority Date: 06/09/1969
• Status: Expired due to Term

• Current Assignee: Samuel T. Nerenberg
• Original Assignee: Samuel T. Nerenberg
• Inventors: Nerenberg, Samuel T.
• Primary Examiner(s): Mack, John H.
• Assistant Examiner(s): Prescott, A. C.
• Application Number:
  US04/831,509
• Time in Patent Office: 974 Days
• Field of Search: 204/18G,299,195P 210/31C,198C
• US Class Current: 204/615
• CPC Class Codes: G01N 27/44704 Details; Accessories

Abstract

A device for adapting an upstanding column to separate macromolecules in preparative amounts by means of electrophoresis, chromatography and electrochromatography carried out in a granular or nongranular gel medium disposed in the column. One device contains an electrically conductive, selfsupporting gel disposed within a tubular housing adapted to sealingly engage the lower portion of the column. Channels may be provided in the adapter gel for fluid ingress to the upper gel surface and egress therefrom. A method for forming the adapter including electrophoresis and electrochromatography performed in a single column.

Claims

2. A device as in claim 1 wherein said gel is an electrolyte-entrapping polymer.

3. A device as in claim 2 wherein said polymer is a cross-linked polyacrylamide.

4. A device as in claim 1 wherein said gel is formed from an electrolytic buffer solution containing about 10- 30% acrylamide monomer.

5. The device of claim 1 together with an outlet port in said tubular housing.

6. The device of claim 5 together with a passage from the upper gel surface to a position adjacent said port.

7. The device of claim 5 together with a separate inlet port in said tubular housing.

8. The device of claim 7 together with separate passages from the upper gel surface to positions adjacent said inlet and outlet ports.

9. An apparatus for separating a mixture of macromolecules by means of electrophoresis, chromatography, or electrochromatography, said device comprising upstanding column means containing a separating medium, vertical adapter means sealingly engaged to a mating lower end of said column means, said adapter means comprising a tubular housing means containing an electrically conductive, self-supporting gel means, said gel forming a seal against liquid flow from the column.

10. An apparatus for separating a mixture of macromolecules by means of electrophoresis, chromatography, or electrochromatography, said device comprising upstanding column means containing a separating medium, vertical adapter means sealingly engaged to a mating lower end of said column means, said adapter means comprising a tubular housing means containing an electrically conductive, self-supporting gel means, said gel means containing channel means interconnecting an opening in the wall of said housing with an opening at the top of said gel means wherein liquid flow from the column is directed through said channel means.