REAGENT FOR DETERMINING BLOOD SUGAR

• US 3,653,836 A
• Filed: 08/26/1970
• Issued: 04/04/1972
• Est. Priority Date: 08/27/1969
• Status: Expired due to Term

• Current Assignee: August Wilhelm Wahlefeld, Erich Bernt, Gruber Wolfgang, Werner Dollacker
• Original Assignee: August Wilhelm Wahlefeld, Erich Bernt, Gruber Wolfgang, Werner Dollacker
• Inventors: Dollacker, Werner, Gruber, Wolfgang, Bernt, Erich, Wahlefeld, August Wilhelm
• Primary Examiner(s): Wolk, Morris O.
• Assistant Examiner(s): Marantz, Sidney
• Application Number:
  US05/066,953
• Time in Patent Office: 587 Days
• Field of Search: 23/23B,253TP 252/408
• US Class Current: 436/95
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: Y10T 436/144444 Glucose

Abstract

A color-producing reagent for determining blood sugar comprising o-toluidine, thiourea, an ethylene glycol mono-lower alkyl ether, phthalic acid, and dimethyl formamide as a solubilizing agent, is outstandingly sensitive even to very low blood sugar contents, as in hypoglycaemia, and exhibits prolonged color stability.

Claims

2. A reagent composition as claimed in claim 1 in which water is contained in an Amount of up to 5 percent by volume.

3. Method for the determination of blood sugar which comprises mixing a blood sample with a reagent composition as claimed in claim 1 and measuring the resulting mixture photometrically as an indication of the blood sugar content.

4. A reagent composition as claimed in claim 1 which comprises 0.05 - 2.5 percent by weight thiourea, 5 - 33 percent by weight phthalic acid, 25 - 65 percent by weight dimethyl formamide, 10 -50 percent by weight ethylene glycol mono-lower alkyl ether and 2.5 - 15 percent by weight o-toluidine, based on total weight of composition.

5. A reagent composition as claimed in claim 4 which comprises 0.075 - 0.15 percent by weight thiourea, 20 - 25 percent by weight phthalic acid, 35 - 55 percent by weight dimethyl-formamide, 20 - 40 percent by weight ethylene glycol mono-lower alkyl ether and 7.5 - 10 percent by weight o-toluidine, based on total weight of composition.

6. A reagent composition as claimed in claim 4 wherein the total volume of dimethyl formamide and of alkylene glycol mono-lower alkyl ether does not exceed 150 parts by volume. 7 Method for the determination of blood sugar which comprises mixing a blood sample with a reagent composition as claimed in claim 4 and measuring the resulting mixture photometrically as an indication of the blood sugar content.