METHOD OF DETERMINING HEMOGLOBIN IN BLOOD

• US 3,663,175 A
• Filed: 10/05/1970
• Issued: 05/16/1972
• Est. Priority Date: 10/05/1970
• Status: Expired due to Term

• Current Assignee: Boehringer Mannheim Corporation
• Original Assignee: David T. Santore, Edward L. Depositar, Robert K. Shipley
• Inventors: Depositar, Edward L., Shipley, Robert K., Santore, David T.
• Primary Examiner(s): Wolk, Morris O.
• Assistant Examiner(s): Katz, Elliott A.
• Application Number:
  US05/078,159
• Time in Patent Office: 589 Days
• Field of Search: 23/230 252/408
• US Class Current: 436/66
• CPC Class Codes: G01N 33/721 Haemoglobin

Abstract

A method of determining hemoglobin in blood using Drabkin'"'"''"'"'s reagent wherein acetone is added to the reagent as a loss of color inhibitor and to aid in attaining immediate recovery of the peak absorbance of cyanmeth-hemoglobin.

Claims

2. The method according to claim 1 wherein the test solution contains about 61 X 10 5 moles of potassium ferricyanide, 77 X 10 5 moles of potassium cyanide and 1190 X 10 5 moles of sodium bicarbonate per liter.

3. The method of inhibiting loss of color in a hemoglobin test solution containing about 61 X 10 5 moles of potassium ferricyanide, 77 X 10 5 moles of potassium cyanide and 1,190 X 10 5 moles of sodium bicarbonate per liter, on exposure of said solution to temperatures below 32* F which comprises adding 0.2 to 10 weight percent acetone to said solution.

4. In the determination of the hemoglobin content of blood using a solution containing potassium cyanide, potassium ferricyanide and sodium bicarbonate in which the potassium ferricyanide is reduced to potassium ferrocyanide the improvement comprising adding from 0.2 to 10 weight percent acetone to said solution to assure immediate attainment of the peak absorbance of cyanmeth-hemoglobin in the spectrophotometric determination of the hemoglobin of blood sample.

5. The method of immediately attaining the cyanmeth hemoglobin peak in the spectrophotometric determination of hemoglobin using the cyanmeth-hemoglobin procedure which comprises adding from about 2 1/2 to 126 ml of acetone per liter to the reagent used in making the determination.