METHOD FOR REMOVING LEVULINIC ACID BY MICROORGANISMS

• US 3,663,368 A
• Filed: 12/12/1968
• Issued: 05/16/1972
• Est. Priority Date: 12/18/1967
• Status: Expired due to Term

• Current Assignee: Michio Harada, Takahiro Ueno
• Original Assignee: Michio Harada, Takahiro Ueno
• Inventors: Ueno, Takahiro, Harada, Michio
• Primary Examiner(s): Monacell, A. Louis
• Assistant Examiner(s): Simons, William Andrew
• Application Number:
  US04/783,202
• Time in Patent Office: 1,251 Days
• Field of Search: 99/14,17,98 195/2,4,29,30
• US Class Current: 435/272
• CPC Class Codes: A23J 3/30 by hydrolysis
• CPC Class Codes: A47G 25/72 Garment-pressing devices hot pressing apparatus or machines D06F
• CPC Class Codes: C12P 7/40 containing a carboxyl group including Peroxycarboxylic acids
• CPC Class Codes: Y10S 435/83 Arthrobacter
• CPC Class Codes: Y10S 435/84 Brevibacterium
• CPC Class Codes: Y10S 435/843 Corynebacterium
• CPC Class Codes: Y10S 435/875 Pseudomonas aeruginosa

Abstract

A method for removing levulinic acid from a liquid containing levulinic acid which comprises adding materials containing cells of microorganisms to a liquid containing levulinic acid, said materials containing cells of microorganisms having been obtained by culturing a microorganism having the ability of utilization of levulinic acid in said liquid. Microorganisms employed include Arthrobacter simplex ATCC 6946, Brevibacterium linens ATCC 21330, Corynebacterium equi ATCC 21329 and Pseudomonas aeruginosa ATCC 21328.

Claims

2. A method according to claim 1, wherein the microorganism is Arthrobacter simplex ATCC 6946, Brevibacterium linens ATCC 21330, Corynebacterium equi ATCC 21329 or Pseudomonas aeruginosa ATCC 21328.

3. A method according to claim 2, wherein preculture of said microorganism iS added to said liquid containing levulinic acid in an amount of 0.5 - 2.0 percent (by volume) and cultured at 20* - 40* C for 20 - 120 hours under aerobic conditions.

4. A method according to claim 2 wherein the pH is between 6.5 and 8.4.

5. A method according to claim 4 wherein the pH is between 7.2 and 7.6.

6. A method according to claim 1, wherein said materials containing cells of microorganisms are seed-cultured liquid, intact cells or dried matters.

7. A method according to claim 6, wherein said intact cells or said dried matters are added to said liquid containing levulinic acid in an amount of 10 - 200 percent (be weight) based on levulinic acid in said liquid and shaken at 20* 40* C for 2 - 5 hours.

8. A method according to claim 1, wherein concentration of levulinic acid in said liquid containing levulinic acid is 3 percent by weight or less, the pH thereof being adjusted to 6.0 -9.0.

9. A method according to claim 1 wherein said liquid containing levulinic acid is an acid hydrolysate of carbohydrate.

10. A method according to claim 9 wherein said acid hydrolysate is acid hydrolysate of a mixture of carbohydrate and crude protein material.