TEST FOR THYROID HORMONE

• US 3,666,854 A
• Filed: 07/30/1969
• Issued: 05/30/1972
• Est. Priority Date: 07/30/1969
• Status: Expired due to Term

• Current Assignee: Anna M. Eisentraut
• Original Assignee: Anna M. Eisentraut
• Inventors: Eisentraut, Anna M.
• Primary Examiner(s): Padgett, Benjamin R.
• Application Number:
  US04/846,289
• Time in Patent Office: 1,035 Days
• Field of Search: 424/1,111,357 23/230 250/16T
• US Class Current: 436/500
• CPC Class Codes: G01N 33/78 Thyroid gland hormones , e.g. T3, T4, TBH, TBG or their receptors
• CPC Class Codes: Y10S 436/804 Radioisotope, e.g. radioimmunoassay
• CPC Class Codes: Y10S 436/808 Automated or kit
• CPC Class Codes: Y10S 436/825 Pretreatment for removal of interfering factors from sample
• CPC Class Codes: Y10S 436/826 Additives, e.g. buffers, diluents, preservatives

Abstract

A procedure utilized in a diagnostic test for determining the level of thyroid hormone within a body fluid which includes admixing a radioactive isotope labeled hormone with a body fluid, and separating the resulting unbound hormone from the hormone bound by the hormone binding globulin and protein of the fluid by sorption of the unbound hormone by a particulate inorganic crystalline material. This sorption does not depend upon critical time or temperature limitations, and is generally conducted by thoroughly admixing the sorbent material with the solution containing the bound and unbound thyroid hormone at room temperature. The sorbent material is then separated from the resultant supernatant fluid and either the supernatant fluid or sorbent material, preferably the sorbent material, is counted with a scintillation counter to determine the labeled hormone bound to or free from the globulin.

Claims

2. The improved test of claim 1 wherein the said particulate inorganic crystalline sorbent material comprises colloidal magnesium silicate.

3. The improved test of claim 2 wherein said sorbing occurs at a temperature within the range of from about 20* C to about 30* C.

4. The method of claim 3 wherein said sorbing occurs during a time interval of at least about 2 minutes.

5. The improved test of claim 4 wherein said sample fluid comprises blood.

6. A method for determining the level of thyroid hormone in a sample of body fluid containing an unknown quantity of said hormone and hormone binding globulin comprising:

a. thoroughly admixing a solution of this sample with a solution containing a radioactive isotope labeled hormone and barbital and allowing the resulting solution to equilibrate;

b. thoroughly admixing the resulting equilibrated solution from step (a) with a particulate inorganic crystalline sorbent material selected from silicic acid and carbonates, phosphates, oxides, hydroxides, silicates, aluminates, and sulfates of the metallic elements in Groups IA, IIA, IIIA, IIB, and VIII of the Periodic Table, and mixed salts thereof to remove free thyroid hormone from said solution by selective sorption thereof by said sorbent material;

c. separating said sorbent from said solution; and

d. counting with a scintillation counter one of (1) the free radioisotope labeled hormone in said sorbent, and (2) the bound radioisotope labeled hormone in said resulting solution.

7. The method of claim 7 wherein said sorbent material is colloidal magnesium silicate.

8. A method of measuring thyroxine in a sample solution comprising:

a. precipitating proteins from said sample solution by admixing said sample solution with an organic solvent and thereby leaving said thyroxine in solution;

b. thoroughly admixing a solution of the thyroxine from step (a) with a solution containing thyrobinding globulin, radioactive isotope labeled thyroxine, and barbital and allowing the resulting solution to equilibrate;

C. thoroughly admixing the equilibrated solution of step (b) with a particulate inorganic crystalline sorbent material selected from silicic acid and carbonates, phosphates, oxides, hydroxides, silicates, aluminates, and sulfates of the metallic elements of Groups IA, IIA, IIIA, IIB and VIII of the Periodic Table, and mixed salts thereof to remove free thyroxine therefrom by selective sorption thereof by said sorbent material;

d. separating said sorbent material from the resulting solution; and

C. counting with a scintillation counter one of (1) free labeled thyroxine removed in said sorbent, and (2) labeled thyroxine remaining in said solution.

9. The method of claim 8 wherein said sorbent material is colloidal magnesium silicate.