LIGAND DETERMINATION WITH SPIN LABELED COMPOUNDS BY RECEPTOR DISPLACEMENT
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Abstract
ORGANIC COMPOUNDS OF BIOLOGICAL INTEREST CAN BE DETERMINED A EXTREMELY LOW CONCENTRATIONS BY COMBINING IN A MEDIUM, THE COMPOSITION TO BE DETERMINED, HEREINAFTER REFEREED TO AS LIGAND, A HIGH MOLECULAR WEIGHT MATERIAL OF AT LEAST 10,000 MOLECULAR WEIGHT, WHICH HAS A SITE SPATIALLY CHARACTERISITIC OF THE LIGAND, HEREINAFTER REFERRED TO AS RECEPTOR, AND AN ANALOG OF THE LIGAND HAVING A FREE RADICAL FUNCTIONALITY, HEREINAFTER REFERRED TO AS "LIGAND ANALOG." THE LIGAND ANALOG AND LIGAND IN THE MEDIUM COMPETE FOR THE RECEPTOR SITE, THE AMOUNT OF LIGAND ANALOG BOND TO THE RECEPTOR, BEING DEPENDENT ON
THE AMOUNT OF LIGAND PRESENT IN THE MEDIUM. BY FOLLOWING THE CHANGE IN ELECTRON SPIN RESONANCE SPECTRUM OF THE LIGAND ANALOG AND COMPARING IT TO THE CHANGE IN SPECTRUM WHICH WOULD BE OBTAINED IN THE ABSENCE OF ANY LIGAND, THE AMOUNT OF LIGAND CAN BE DETERMINED. COMPOUNDS ARE PROVIDED WHICH ARE LIGAND ANALOGS FOR USE IN THE ASSAY.
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