OPIUM ALKALOID ANTIGENS AND ANTIBODIES SPECIFIC THEREFOR

• US 3,709,868 A
• Filed: 03/08/1971
• Issued: 01/09/1973
• Est. Priority Date: 03/08/1971
• Status: Expired due to Term

• Current Assignee: Sidney Spector
• Original Assignee: Sidney Spector
• Inventors: Spector, Sidney
• Primary Examiner(s): Schain, Howard E.
• Application Number:
  US05/122,204
• Time in Patent Office: 673 Days
• Field of Search: 424/85 260/112,119,121
• US Class Current: 530/363
• CPC Class Codes: C07D 489/02 with oxygen atoms attached in positions 3 and 6, e.g. morphine, morphinone
• CPC Class Codes: G01N 33/9486 Analgesics, e.g. opiates, aspirine
• CPC Class Codes: Y10S 436/803 Stabe free radicals, e.g. spin immunoassay
• CPC Class Codes: Y10S 436/804 Radioisotope, e.g. radioimmunoassay
• CPC Class Codes: Y10S 436/816 Alkaloids, amphetamines, and barbiturates
• CPC Class Codes: Y10S 436/822 Identified hapten
• CPC Class Codes: Y10S 436/823 Immunogenic carrier or carrier per se
• CPC Class Codes: Y10S 436/824 Immunological separation techniques
• CPC Class Codes: Y10S 530/806 Antigenic peptides or proteins
• CPC Class Codes: Y10S 530/807 Hapten conjugated with peptide or protein

Abstract

Opium alkaloid antigens are prepared by coupling opium alkaloid haptens to immunogenic carrier materials. In preferred embodiments proteins are used as carrier materials and the coupling is effected through the carboxyl group of a carboxy lower alkyl derivative of the phenolic hydroxy group of said alkaloid and a free amino group on the protein thereby yielding a covalent peptide bond. The resulting antigens produce immunological effects when injected into host animals, including the formation of opium alkaloid specific antibodies. These specific antibodies are useful in bioanolytical techniques for the assay of opium alkaloids in biological fluids.

Claims

2. The antigen of claim 1 wherein said opium alkaloid derivative is 3-0-carboxymethylmorphine.

3. The antigen of claim 1 wherein said protein is bovine serum albumin.

4. The antigen of claim 3 wherein three to seven opium alkaloid derivative groups are bonded per molecule of bovine serum albumin.

5. An antigen consisting essentially of a carboxy lower alkyl derivative of the phenolic hydroxyl group of an opium alkaloid bonded through a peptide linkage to an amino group of an immunogenic carrier material selected from the group consisting of proteins and polypeptides.