METHOD OF CELL CULTURE

• US 3,717,551 A
• Filed: 07/02/1970
• Issued: 02/20/1973
• Est. Priority Date: 07/09/1969
• Status: Expired due to Term

• Current Assignee: Bernard Bizzini, Claude Jasmin, Jean-Claude Chermann, Marcel Raynaud
• Original Assignee: Bernard Bizzini, Claude Jasmin, Jean-Claude Chermann, Marcel Raynaud
• Inventors: Jasmin, Claude, Bizzini, Bernard, Chermann, Jean-Claude, Raynaud, Marcel
• Primary Examiner(s): Huff, Richard L.
• Application Number:
  US05/052,062
• Time in Patent Office: 964 Days
• Field of Search: 424/89 195/1.1,1.7,1.8 167/78,79
• US Class Current: 435/403
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: C12N 2531/00 Microcarriers
• CPC Class Codes: C12N 2533/10 Mineral substrates
• CPC Class Codes: C12N 2740/13051 Methods of production or purification of viral material
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: Y10S 435/882 Staphylococcus

Abstract

A method for the culture of cells of the type which grow in films and substantially in monolayers, the method being intended to be employed for virus production, the cells to be cultivated being fixed on porous silica spherules which are maintained in suspension in the culture medium.

Claims

1. A method of culture of cells intended for virus production and of the type which grow in a monolayer film, wherein the cells to be cultivated are fixed on porous silica spherules, having diameters of about 125 microns, which are maintained in suspension in the culture medium, the mean diameter of the pores of said spherules being within the 100 - 1350 A-range.