METHOD FOR DETERMINING THE PRESENCE OF REAGIN-IMMUNOGLOBULINS(REAGIN-IG)DIRECTED AGAINST CERTAIN ALLERGENS,IN AQUEOUS SAMPLES

• US 3,720,760 A
• Filed: 09/06/1968
• Issued: 03/13/1973
• Est. Priority Date: 09/06/1968
• Status: Expired due to Term

• Current Assignee: Pharmacia AB (Pharmacia Corp.)
• Original Assignee: Hans H. Bennich, Johansson Stig G O, Leif E. Wide
• Inventors: Wide, Leif E., Bennich, Hans H., JOHANSSON STIG G O
• Application Number:
  US04/758,131
• Time in Patent Office: 1,649 Days
• US Class Current: 436/513
• CPC Class Codes: C07K 16/4283 against an allotypic or isotypic determinant on Ig
• CPC Class Codes: G01N 33/544 the carrier being organic
• CPC Class Codes: Y10S 436/804 Radioisotope, e.g. radioimmunoassay
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures

Abstract

AN IN VITRO METHOD FOR ANALYZING A BODY FLUID FOR REAGIN-IMMUNOGLOBULINS THEREIN THAT ARE REACTIVE WITH ALLERGENS WHICH COMPRISES CONTACTING A SAMPLE OF BODY FLUID WITH A WATER-INSOLUBLE POLYMER TO WHICH IS ATTACHED AT LEAST ONE TEST ALLERGEN FOR A LONG ENOUGH TIME TO ALLOW REACTION THEREBETWEEN AND THEN CONTACTING WITH EITHER LABELLED ANTIBODIES THAT ARE REACTIVE WITH THE REAGIN IMMUNOGLOBULINS OR WITH ANTIBODIES AGAINST REAGIN-IG AND REAGIN-IG LABELLED WITH A RADIATION EMITTING ATOM OR GROUP, SEPARATING INTO A LIQUID PHASE AND A SOLID PHASE, AND MEASURING THE RADIATION EMITTED FROM AT LEAST ONE OF THE TWO SEPARATED PHASES.

Claims