MICROSCOPE SLIDE ASSEMBLY

• US 3,736,042 A
• Filed: 05/05/1971
• Issued: 05/29/1973
• Est. Priority Date: 05/05/1971
• Status: Expired due to Term

• Current Assignee: Arthur L. Markovits, Stanley W. Wolfson
• Original Assignee: Arthur L. Markovits, Stanley W. Wolfson
• Inventors: Markovits, Arthur L., Wolfson, Stanley W.
• Primary Examiner(s): Rubin, David H.
• Application Number:
  US05/140,497
• Time in Patent Office: 755 Days
• Field of Search: 350/92-95 356/244-246 117/124E 24/253TP
• US Class Current: 359/398
• CPC Class Codes: G01N 1/28 Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Qmounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 ; laboratory gas handling apparatus B01L5/00
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G02B 21/34 Microscope slides, e.g. mounting specimens on microscope slides

Abstract

A microscope slide assembly and a method of preparing such which includes an optically clear glass slide having a controlled thickness fluorocarbon layer coated on one planar surface. The layer is provided with a multiplicity of openings to form wells on the glass slide. An agar solution may be incorporated within the wells to provide an adhesive layer. In addition, a particular antigen may be layered on top of the agar composition to provide a completed assembly wherein only a patient'"'"''"'"'s serum is needed to process a microscopic examination. For use in fluoroescent microscopy, a film body grade Teflon containing an adhesive is used to provide the uniform fluorocarbon layer. The microscopic assembly may be enclosed within an environment isolation container to prevent contamination of the assembly from the ambient surroundings when the antigen is combined with the agar solution.

Claims

2. The microscope slide system as recited in claim 1 wherein said plurality of openings include a predetermined area bounded by said fluorocarbon layer in a continuous manner, each of said plurality of openings being non-contiguous with respect to an adjacent opening.

3. The microscope slide system as recited in claim 1 wherein said fluorocarbon layer thickness is substantially equal to 1 X 10 4 inches.

4. The microscope slide system as recited in claim 1 wherein said fluorocarbon layer comprises a film bonding grade fluorocarbon having an adhesive for securing said layer to said boundary surface of said glass slide.

5. The microscope slide system as recited in claim 1 wherein said fluorocarbon layer includes a uniform predetermined thickness contiguous to said slide surface, said fluorocarbon layer being substantially composed of a tetrafluorethylene fluorocarbon resin.

6. The microscope slide system as recited in claim 5 wherein said thickness is substantially equal to 1 X 10 4 inches.

7. The microscope slide system as recited in claim 1 wherein said plurality of openings define a plurality of areas on said surface of said slide, said areas having an adhesive layer deposited thereon.

8. The microscope slide system as recited in claim 7 wherein said adhesive layer is composed of agar.

9. The microscope slide system as recited in claim 7 wherein said adhesive layer is composed of a .01% to .05% agarose solution.

10. The microscope slide system as recited in claim 9 wherein said adhesive layer is substantially composed of a .02% agarose solution.

11. The microscope slide system as recited in claim 10 wherein each of said plurality of areas includes a predetermined antigen layer deposited on an upper surface of said adhesive layer.

12. A fluorescent microscopy assembly, comprising:

a. a microscope slide having a planar contour dimension with opposing boundary surfaces, one of said boundary surfaces having a fluorocarbon layer fixedly deposited thereon, said layer having a predetermined thickness within an approximating range between 7.5 X 10 5 and 1.5 X 10 4 inches, said fluorocarbon layer further having a plurality of openings passing therethrough for forming reactant regions contiguous to said boundary surface of said slide; and

, b. enclosing means for said microscope slide, said enclOsing means having an external surface substantially impervious to moisture for isolating said slide from an external environment, said enclosing means comprising a pair of adjacently positioned elements fastened each to the other on a common boundary periphery to form a total enclosure for said microscope slide.

13. The fluorescent microscopy assembly as recited in claim 12 wherein said plurality of openings of said fluorocarbon layer define a multiplicity of apertures passing through said fluorocarbon layer to said boundary surface contiguous with said layer.

14. The fluorescent microscopy assembly as recited in claim 13 wherein each of said multiplicity of apertures defines a reactant region bounded by said fluorocarbon layer in a continuous manner.

15. The fluoroescent microscopy assembly as recited in claim 14 wherein each of said multiplicity of apertures is non-contiguous with respect to an adjacent aperture.

16. The fluoroescent microscopy assembly as recited in claim 12 wherein said fluorocarbon layer thickness is substantially equal to 1 X 10 4 inches.

17. The fluoroescent microscopy assembly as recited in claim 12 wherein said plurality of openings define a plurality of areas on said surface of said slide, said areas having an adhesive layer deposited thereon, said deposited ashesive layer defining an upper surface plane.

18. The fluorescent microscopy assembly as recited in claim 17 wherein said adhesive layer is composed of agar.

19. The fluorescent microscopy assembly as recited in claim 17 wherein said adhesive layer is composed of a .01% to .05% agarose solution.

20. The fluorescent microscopy assembly as recited in claim 17 wherein said adhesive layer is substantially composed of a .02% agarose solution.

21. The fluorescent microscopy assembly as recited in claim 17 wherein each of said plurality of areas includes a predetermined antigen layer deposited on said upper surface plane of said adhesive layer.

22. The fluorescent microscopy assembly as recited in claim 21 wherein said deposited antigen and adhesive layers are confined to said reactant regions.

23. The fluorescent microscopy assembly as recited in claim 22 wherein said adhesive layer is substantially composed of a .02% agarose solution.

24. The fluorescent microscopy assembly as recited in claim 23 wherein said .02% agarose solution layer and said predetermined antigen layer are deposited only within said reactant regions.

25. The fluorescent microscopy assembly as recited in claim 12 wherein said fluorocarbon layer is substantially composed of a tetrafluorethylene fluorocarbon resin.

26. The fluorescent microscopy assembly as recited in claim 25 wherein said layer has a thickness substantially equal to 1 X 10 4 inches.

27. The fluorescent microscopy assembly as recited in claim 12 wherein said external surface of said enclosing means is formed of an aluminum foil.

28. The fluorescent microscopy assembly as recited in claim 12 wherein said external surface of said enclosing means is formed of moisture impervious plastic sheeting.

29. The fluorescent microscopy assembly as recited in claim 12 wherein said enclosing means defines an inner volume having an inert gas for presenting an internal inert environment to said enclosed microscope slide.

30. The fluorescent microscopy assembly as recited in claim 12 wherein said enclosing means includes a desiccant for insertion adjacent said slide for preserving a moisture free environment within said enclosing means.