ARTIFICIAL INSEMINATION OF SOWS

• US 3,791,384 A
• Filed: 05/11/1972
• Issued: 02/12/1974
• Est. Priority Date: 07/15/1971
• Status: Expired due to Term

• Current Assignee: Albert Liedicke, Ludwig Richter
• Original Assignee: Albert Liedicke, Ludwig Richter
• Inventors: Richter, Ludwig, Liedicke, Albert
• Primary Examiner(s): Medbery, Aldrich F.
• Application Number:
  US05/252,272
• Time in Patent Office: 642 Days
• Field of Search: 128/1,260 195/1.8 424/89 62/1
• US Class Current: 600/35
• CPC Class Codes: A01K 29/00 Other apparatus for animal husbandry

Abstract

A method of preserving boar sperm by deep-freezing thawing and introduction into the uterus of the sow, in which method the ejaculate after being collected is centrifuged, and the sediment is diluted, frozen to a temperature of -180* to -200*C, at the time of use is thawed by being placed in such a quantity of diluent warmed to 40* to 50* C that a dilution of the sperm which is appropriate for effective bathing of the uterus is achieved, and the diluted fluid is introduced into the uterus. The ejaculate is inactivated after being collected and before being centrifuged, and after being thawed the sperm solution has added to it a solution which mobilizes the spermatozoa.

Claims

1. The method of impregnating a sow comprising the steps of immobilizing the spermatozoa of boar semen with a first diluting spermatozoa inactivating solution, centrifuging the diluted semen to produce a sperm sediment, diluting the sperm sediment with a freezing diluent, slowing cooling the resultant sperm solution to approximately 5* C, freezing and storing the cooled sperm solution at a temperature of approximately -180* C to -200* C, thawing the frozen sperm solution when needed, mixing the thawed sperm solution with a spermatozoa activating solution and utilizing said activated sperm solution within the uterus of a sow.

2. In the method of impregnating a sow as in claim 1 wherein said first diluting solution and said freezing diluent contain glucose and a buffer system.

3. In the method of impregnating a sow as in claim 1 wherein the thawing of the frozen sperm solution, mixing with an activating solution and utilization within the uterus of a sow comprises the steps of thawing the frozen sperm solution within a spermatozoa diluting solution, introducing the thawed diluted solution within the uterus of a sow, and introducing a spermatozoa activating solution into the uterus of the sow to activate the spermatozoa therein.

4. In the method of impregnating a sow as in claim 1 wherein said boar semen is diluted by said first solution immediately upon collection and said first solution is warmed to approximately 36* C.

5. In the method of impregnating a sow as in claim 4 wherein said first solution is slowly cooled to approximately room temperature prior to centrifuging.

6. In the method of impregnating a sow as in claim 1 wherein said resultant sperm solution is maintained at said approximate 5* C from 1 1/2 to 2 hours and is then dropped in droplets upon CO2 dry ice to form solid pellets, said pellets being stored by immersion in liquid nitrogen.

7. In the method of impregnating a sow as in claim 1 wherein the step of thawing the frozen sperm solution comprises mixing the frozen sperm solution with a heated diluting solution.

8. In the method of impregnating a sow as in claim 7 wherein said heated diluting solution is of an initial temperature of approximately 60* C to 70* C.

9. In the method of impregnating a sow as in claim 7 wherein said thawing of said frozen sperm solution is made with an amount of heated diluting solution insufficient to obtain a desired final dilution, and the desired final dilution is obtained by introducing the thawed prediluted solution into the uterus of the sow and thereafter introducing a sufficient amount of said heated diluting solution into the uterus of the sow to obtain the desired final dilution of activated sperm solution.

10. In the method of impregnating a sow as in claim 7 wherein said heated diluting solution inactivates the spermatozoa at room temperature but activates the spermatozoa at the sow'"'"''"'"'s body temperature.

11. In the method of impregnating a sow as in claim 7, characterized in that said solutions used for spermatozoa activation and inactivation contain glucose, sodium citrate, sodium bicarbonate, antibiotics, ethylenediaminetetraacetic acid and potassium chloride, and said freezing diluent contains skimmed milk powder, glucose, egg yolk, glycerine, antibiotics, ethylenediaminetetrAacetic acid and potassium chloride.

12. In the method of impregnating a sow as in claim 11 wherein said spermatozoa activating and inactiviating solutions contain about 100 to 140 g, preferably about 120 g, glucose, about 10 to 15 g, preferably about 12 g, sodium citrate, about 1 to 3 g, preferably about 2 g, sulfanilamide, about 0.5 to 3 g, preferably about 1.5 g, potassium chloride, about 1.5 to 4 g, preferably about 2.5 g, sodium bicarbonate, about 1.4 to 4 g, preferably about 2.5 g, ethylenediaminetetraacetic acid, about 1.7 millions i. u. penicillin G sodium, about 1.7 i.u. dihydrostreptomycin base in 2,000 ml water, and is adjusted be means of 4% NaOH to a pH of about 6.8.

13. In the method of impregnating a sow as in claim 11 wherein said freezing diluent contains about 100 to 120 g, preferably about 100 g, skimmed milk powder, about 20 to 30 g, preferably about 25 g, glucose, about 0.5 to 3 g, preferably about 1 g, ethylenediaminetetraacetic acid, about 0.5 to 1.5 g, preferably about 0.8 g, potassium chloride, about 1 million i.u. penicillin G sodium, about 1 million i.u. dihydrostreptomycin base, about 180 ml egg yolk, and about 60 ml glycerine per 1000 ml of water.