AUTOMATED DIRECT METHOD FOR THE DETERMINATION OF INORGANIC PHOSPHATE IN SERUM

• US 3,795,484 A
• Filed: 12/22/1971
• Issued: 03/05/1974
• Est. Priority Date: 12/22/1971
• Status: Expired due to Term

• Current Assignee: Baker Instruments Corporation
• Original Assignee: Gerhard Ertingshausen, John A. Daly
• Inventors: Daly, John A., Ertingshausen, Gerhard
• Primary Examiner(s): Scovronek, Joseph
• Application Number:
  US05/211,075
• Time in Patent Office: 804 Days
• Field of Search: 23/23B,23R,253R,259 250/218 356/197,246,196,204
• US Class Current: 436/46
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH
• CPC Class Codes: Y10T 436/112499 with sample on test slide
• CPC Class Codes: Y10T 436/166666 of inorganic phosphorus compound in body fluid

Abstract

A direct method is provided for the spectrophotometric determination of inorganic phosphate in fluids, particularly body fluids, such as blood serum. The process requires only a single reagent addition and comprises reacting the phosphate-containing fluid with an ammonium molybdate solution and thereafter measuring the absorbance within a specified time interval before the reaction has measurably proceeded and at the end of the reaction by means of a centrifugal analytical photometer. Inasmuch as a linear relationship exists between the phosphate concentration and the change of absorbance, the concentration is an unknown sample can be conveniently calculated by comparison with the results obtained from the simultaneous measurement of a sample of known concentration.

Claims

2. The process of claim 1 wherein said fluid is a body fluid.

3. The process of claim 1 wherein said fluid is blood serum.

4. The process of claim 1 wherein said mixture contains a surface active agent.

5. The process of claim 4 wherein said surface active agent is a sorbitan monooleate.