ENZYME AMPLIFICATION ASSAY

• US 3,817,837 A
• Filed: 11/06/1972
• Issued: 06/18/1974
• Est. Priority Date: 05/14/1971
• Status: Expired due to Term

• Current Assignee: Rubenstein K, Ullman E
• Original Assignee: Rubenstein K, Ullman E
• Inventors: RUBENSTEIN K, ULLMAN E
• Application Number:
  US05/304,156
• Time in Patent Office: 589 Days
• US Class Current: 435/7.9
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/946 CNS-stimulants, e.g. cocaine, amphetamines
• CPC Class Codes: G01N 33/948 Sedatives, e.g. cannabinoids, barbiturates opiates G01N33/9486
• CPC Class Codes: G01N 33/9486 Analgesics, e.g. opiates, aspirine
• CPC Class Codes: Y10S 930/26 Containing cys-cys disulfide bridge between nonadjacent cysteine residues

Abstract

NOVEL BIOLOGICAL ASSAY METHOD FOR DETERMINING THE PRESENCE OF A SPECIFIC ORGANIC MATERIAL BY EMPLOYING A MODI-SPECIFIC FOR ONE OR A GROUP OF MATERIALS (HEREINAFTER REFERRED AS TO "LIGANDS") AND BINDING AN ENZYME TO THE LIGAND OR LIGANF.
D C UNTERFEIT TO PROVIDE AN "ENZYME-BOUNDLIGAND OR LIGANG COUNTERFEIT TO PROVIDE AN "ENZYME-BOUNDLIGAND," AN EXTREMELY SENSITIVE METHOD IS PROVIDED FOR ASSAYING FOR LIGANDS. THE RECEPTOR WHEN BOUND TO THE ENZYME-BOUND-LIGAND SUBSTANTIALLY INHIBITS ENZYMATIC ACTIVITY, PROVIDING FOR DIFFERENT CATALYTIC EFFICIENCIES OF ENZYME-BOUND-LIGAND AND ENZYME-BOUND-LIGAND COMBINED WITH RECEPTOR. THE RECEPTOR, LIGAND AND ENZYME-BOUND-LIGAND ARE COMBINED IN AN ARBITRARY ORDER AND THE EFFECT OF THE PRESENCE OF LIGAND ON ENZYMATIC ACTIVITY DETERMINED. VARIOUS PROTOCOLS MAY BE USED FOR ASSAYING FOR ENNZYMATIC ACTIVITY AND RELATING TO THE RESULT TO THE AMOUNT OF LIGAND PRESENT.

Claims