DIAGNOSTIC TEST STRIP FOR THE DETECTION OF COMPONENTS OF BODY FLUIDS

• US 3,853,472 A
• Filed: 07/03/1973
• Issued: 12/10/1974
• Est. Priority Date: 07/18/1972
• Status: Expired due to Term

• Current Assignee: Hans-George Rey, Peter Rieckmann, Walter Rittersdorf, Werner Guthlein
• Original Assignee: Hans-George Rey, Peter Rieckmann, Walter Rittersdorf, Werner Guthlein
• Inventors: Rittersdorf, Walter, Guthlein, Werner, Rieckmann, Peter, Rey, Hans-George
• Primary Examiner(s): Wolk, Morris O.
• Assistant Examiner(s): Turk, Arnold
• Application Number:
  US05/376,248
• Time in Patent Office: 525 Days
• Field of Search: 23/23B,253TP 252/408,186,4A 195/13.5R
• US Class Current: 436/66
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: Y10S 436/904 Oxidation - reduction indicators

Abstract

Test strips are provided for detecting even small amounts of blood or other peroxidatively active substances in body fluids; the strips comprising a carrier containing a hydroperoxide, a chromogen, and as an activator, a compound of the formula WHEREIN R1 is a hydrogen atom or a methyl radical and benzene and/or pyridine rings are fused on at least one of the positions indicated with c, (d,e), f and g, provided that two adjacent rings must not simultaneously each contain a cyclic nitrogen atom, and wherein the aromatic compounds (1), apart from the positions indicated by H and R1, can be substituted by lower alkyl radicals which together can also form a hydroaromatic ring.

Claims

1. TEST STRIP FOR THE DETECTION OF PEROXIDATIVELY-ACTIVE SUBSTANCES IN BODY FLUID, COMPRISING A CARRIER CONTAINING A HYDROPEROXIDE, AT LEAST ONE CHROMOGEN AND, AS AN ACTIVATOR, A COMPOUND OF THE FORMULA

2. Test strip as claimed in claim 1, wherein R1 is formula (I) is hydrogen.

3. Test strip as claimed in claim 1, wherein R1 in formula (I) is methyl.

4. Test strip as claimed in claim 1, wherein said compound contains a benzene ring fused on at least one of the c, (d,e), f or g positions.

5. Test strip as claimed in claim 1, wherein said compound contains a pyridine ring fused on at least one of the f or g positions.

6. Test strip as claimed in claim 1, wherein said compound contains a benzene ring fused to at least one of the c, (d,e), f or g positions and a pyridine ring on one of the f and g positions.

7. Test strip as claimed in claim 1, wherein said compound is substituted in at least one available ring position by a lower alkyl radical of from one to four carbon atoms.

8. Test strip as claimed in claim 7, wherein there are two such alkyl radicals linked together to form a 5 or 6-membered hydroaromatic ring.

9. Test strip as claimed in claim 1, wherein said compound is a benzo (c) quinoline.

10. Test strip as claimeD in claim 1, wherein said compound is a benzo (f) quinoline.

11. Test strip as claimed in claim 1, wherein said compound is a benzo (g) quinoline.

12. Test strip as claimed in claim 1, wherein said compound is a dibenzoquinoline.

13. Test strip as claimed in claim 12, wherein said dibenzoquinoline is dibenzo (c,f) quinoline.

14. Test strip as claimed in claim 12, wherein said dibenzoquinoline is dibenzo (c,d,e) quinoline.

15. Test strip as claimed in claim 1, wherein said compound is a pyridoquinoline.

16. Test strip as claimed in claim 15, wherein said pyridoquinoline is a pyrido (2,3-f) quinoline.

17. Test strip as claimed in claim 16, wherein said pyridoquinoline is a pyrido (3,2-f) quinoline.

18. Test strip as claimed in claim 16, wherein said pyridoquinoline is a pyrido (2,3-g) quinoline.

19. Test strip as claimed in claim 1, wherein said compound is phenanthridine.

20. Test strip as claimed in claim 1, wherein said compound is selected from the group consisting of 2-methyl- or 2-ethyl-phenanthridine benzo (f) quinoline 1,2-tetramethylenebenzo (f) quinoline benzo (g) quinoline 4-methylbenzo (g) quinoline dibenzo (c,d,e) quinoline dibenzo (c,f) quinoline pyrido (3,2-f) quinoline 3-methyl-4,7-phenanthroline pyrido (2,3-f) quinoline 3-methylbenzo (f) quinoline 1,3-dimethylbenzo (f) quinoline 2,4-dimethylbenzo (g) quinoline 6-methylphenanthridine 3,8-dimethyl-4,7-phenanthroline 2,8-dimethyl-1,7-phenanthroline, and 2,7-dimethyl-1,6-anthrazoline

21. Test strip as claimed in claim 1, wherein the carrier is an absorbent material impregnated with the reagents.

22. Test strip as claimed in claim 1, wherein the carrier is a water-stable film with the reagents incorporated therein.

23. Test strip as claimed in claim 1, wherein the reagent solution used for the production thereof contains 0.05-1 percent of activator per 100 ml. of solution.

24. Test strip as claimed in claim 1, wherein the reagent solution used for the production thereof contains 0.2-0.5 percent of activator per 100 ml. of solution.

25. Test strip as claimed in claim 1, wherein the reagent solution used for the production thereof contains 0.05-5 g. of chromogen per 100 ml. of solution.

26. Test strip as claimed in claim 1, wherein the reagent solution used for the production thereof contains 0.2-1.0 g. of chromogen per 100 ml. of solution.

27. Test strip as claimed in claim 1, wherein the reagent solution used for the production thereof contains a buffer and or a complex former, a thickener or a wetting agent.

28. Method of detecting small amounts of blood in a body fluid which method comprises contacting a test sample of the body fluid with a test strip as claimed in claim 1 and observing the color formation thereon as an indication of the absence or presence of blood in said saple.

29. Method of detecting small amounts of peroxidatively active substances in a body fluid which method comprises contacting a test sample of the body fluid with a test strip as claimed in claim 1 and observing the color formation thereon as an indication of the absence or presence of peroxidatively active substances in said sample.