LATEX POLYMER REAGENTS FOR DIAGNOSTIC TESTS

• US 3,857,931 A
• Filed: 01/17/1972
• Issued: 12/31/1974
• Est. Priority Date: 02/01/1971
• Status: Expired due to Term

• Current Assignee: Hans Jacob Hager
• Original Assignee: Hans Jacob Hager
• Inventors: Hager, Hans Jacob
• Primary Examiner(s): Meyers, Albert T.
• Assistant Examiner(s): Fagelson, A. P.
• Application Number:
  US05/218,481
• Time in Patent Office: 1,079 Days
• Field of Search: 424/3,11,12,13,78,79,81,87,92 260/112R,112B,8
• US Class Current: 436/509
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form
• CPC Class Codes: G01N 33/571 for venereal disease, e.g. syphilis, gonorrhoea herpes G01N33/56994; chlamydia G01N33/56927
• CPC Class Codes: Y10S 436/818 Human chorionic gonadotropin
• CPC Class Codes: Y10S 530/806 Antigenic peptides or proteins
• CPC Class Codes: Y10S 530/816 Attached to the carrier via a bridging agent
• CPC Class Codes: Y10S 530/868 involving autoimmunity, allergy, immediate hypersensitivity, delayed hypersensitivity, immunosuppression, or immunotolerance

Abstract

Diagnostically useful reagents formed by chemically combining a serologically determinant material to a polymeric carrier particle utilizing a water-soluble monocarbodiimide as a condensing agent as well as diagnostic methods utilizing the reagents are disclosed.

Claims

1. A WATER-INSOLUBLE IMMUNOLOGICAL DIAGNOSTIC REAGENT HAVING A SPECIFIC GRAVITY OF ABOUT THAT OF WATER COMPRISING DISCRETE PARTICLES OF A SEROLOGICALLY INERT LATEX POLYMER SELECTED FROM THE GROUP CONSISTING OF CARBOXYLATED STYRENE BUTADIENE, CARBOXYLATE POLYSTYRENE, CARBOXYLATE POLYSTYRENE WITH AMINO GROUPS, ACRYLIC ACID POLYMERS, METHACRYLIC ACDI POLYMERS, ACRYLONITRILE BUTADIENE STYRENE, POLYVINYL ACETATE ACRYLATE, POLYVINYL PYRIDINE AND VINYL-CHORIDE ACRYLATE HAVING CONDENSED THERETO, WITH A WATER SOLUBLE CARBODIIMIDE COUPLING AGENT, THROUGH AN AMIDE LINKAGE, FROM ABOUT 0.01 TO ABOUT 15.0% BY WEIGHT OF A KNOWN SEROLOGICALLY DETERMINANT MATERIAL SELECTED FROM THE GROUP CONSISTING OF HUMAN CHORIONIC GONADOTROPIN AND HUMAN GAMMA GLOBULIN.

2. The reagent of claim 1 wherein said serologically inert latex polymer is a water insoluble carboxylated styrene butadiene copolymer having a specific gravity of about that of water and a particle size of about 0.01 to about 0.9 microns.

3. The reagent of claim 1 wherein the serologically determinant material is human chorionic gonadotropin.

4. The reagent of claim 1 wherein the serologically determinant material is denatured human gamma globulin.

5. A method of detecting the presence of chorionic gonadotropin in human body fluids which comprises mixing the test fluid with antiserum for chorionic gonadotropin, then contacting the mixture with an aqueous suspension of the water-insoluble reagent of claim 3 and observing the results.

6. A method of detecting the presence of rheumatoid factor in human body fluids which comprises mixing the test fluid with an aqueous suspension of the water-insoluble reagent of claim 4 and observing the results.