LACTATE DEHYDROGENASE TEST MATERIAL

• US 3,867,259 A
• Filed: 11/08/1973
• Issued: 02/18/1975
• Est. Priority Date: 11/08/1973
• Status: Expired due to Term

• Current Assignee: Il Holding SpA (Werfen SA)
• Original Assignee: Peter Salvatore Forgione
• Inventors: Forgione, Peter Salvatore
• Primary Examiner(s): Tanenholtz, Alvin E.
• Application Number:
  US05/414,035
• Time in Patent Office: 467 Days
• Field of Search: 195/13.5R,99
• US Class Current: 435/26
• CPC Class Codes: C12Q 1/32 involving dehydrogenase
• CPC Class Codes: Y10S 435/805 Test papers

Abstract

A diagnostic test indicator for the determination of the concentration of lactate dehydrogenase in sera comprising a bibulous material which has contained therein the dried residue resulting from the impregnation thereof with 1. A TETRAZOLIUM SALT, 2. A CHROMATOGRAPHIC EFFECT PREVENTOR, 3. AN ANTI-OXIDANT, 4. DIAPHORASE, AND 5. A NICOTINAMIDE-ADENINE-DINUCLEOTIDE- AN ALKALI LACTATE SALT MIXTURE AND A PROCESS FOR THE PRODUCTION OF SAID INDICATOR, ARE DISCLOSED.

Claims

1. A TETRAZOLIUM SALT,

2. A diagnostic test indicator according to claim 1 wherein said (1) is 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride.

3. AN ANTI-OXIDANT,

4. A diagnostic test indicator according to claim 1 wherein said (3) is p-dimethylaminonitrosobenzene.

5. A NICOTINAMIDE-ADENINE-DINUCLEOTIDE-ALKALI LACTATE SALT MIXTURE.

6. A diagnostic test indicator according to claim 1 wherein said alkali lactate salt is lithium lactate.

7. A diagnostic test indicator according to claim 1 wherein the bibulous material additionally contains (6) a carbohydrate stabilizer for said diaphorase.

8. A diagnostic test indicator according to claim 1 wherein the bibulous material additionally contains (7) a nonionic wetting agent.

9. A process for the preparation of the diagnostic test indicator of claim 1 which comprises impregnating a bibulous material with an aqueous solution of said (1) and (2), thereafter drying the thus impregnated material, impregnating the thus impregnated material with an organic solvent solution of said (3), thereafter drying the thus twice impregnated material, impregnating the this twice impregnated material with a solution of said (4), thereafter drying the thrice impregnated material, and then impregnating the thus thrice impregnated material with (5) and drying the thus four times impregnated material.

10. A process according to claim 9 wherein said organic solvent is hexane or chloroform.

11. A process according to claim 9 wherein said (1) is 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl 2H-tetrazolium chloride.

12. A process according to claim 9 wherein said (2) is poly(methacrylic acid).

13. A process according to claim 9 wherein said (3) is p-dimethylaminonitrosobenzene.

14. A process according to claim 9 wherein said (3) is dilauryethiodipropionate.

15. A process according to claim 9 wherein said alkali lactate salt is lithium lactate.

16. A diagnostic test indicator according to claim 1 wherein said bibulous material is paper.

17. A process according to claim 9 wherein said bibulous material is paper.