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DIRECT RADIOIMMUNOASSAY FOR ANTIGENS AND THEIR ANTIBODIES

  • US 3,867,517 A
  • Filed: 12/21/1971
  • Issued: 02/18/1975
  • Est. Priority Date: 12/21/1971
  • Status: Expired due to Term
First Claim
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1. A METHOD FOR DETERMINING THE PRESENCE OF A HEPATITIS ASSOCIATED ANTIGEN OR ITS ANTIBODY CAPABLE OF BEING BOUND TO TWO HEPATITIS ASSOCIATED ANTIGENS OR ANTIBODIES RESPECTIVELY IN AN UNKNOWN SAMPLE UTILIZING DIRECT RADIOMMUNOASSAY COMPRISING:

  • A. FORMING A SOLUTION OF SAID HEPATITIS ASSOCIATED ANTIGEN OR ANTIBODY IN A BUFFER MIXTURE;

    B. COATING A TEST APPARATUS BY CONTACTING SAID TEST APPARATUS WTIH SAID SOLUTION AND C. INCUBATING SAID TEST APPARATUS WHILE IN CONTACT WITH SAID SOLUTION D. WASHING SAID CUBATED COATED TEST APPARATUS WITH A BUFFER MIXTURE;

    E. PLACING SAID UNKNOWN SAMPLE IN CONTACT WITH SAID INCUBATED AND WASHED TEST APPARATUS;

    F. INCUBATING SAID UNKNOWN SAMPLE WHILE IN CONTACT WITH SAID COATED TEST APPARATUS FOR FROM 0.5 TO 42 HOURS TO BOND ANY OF SAID HEPATITIS ASSOCIATED ANTIGEN OR ANTIBODY PRESENT IN SAID UNKNOWN SAMPLE TO SAID INCUBATED TEST APPARATUS;

    G. WASHING SAID INCUBATED TEST APPARATUS WITH A BUFFERED SOLUTION TO REMOVE SAID UNKNOWN SAMPLE LEAVING SAID BONDED HEPATITIS ASSOCIATED ANTIGEN OR ANTIBODY ON THE COATING OF SAID TESPT APPARATUS;

    H. CONTACTING SAID WASHED TEST APPARATUS WITH A PURIFIED FORM OF SAID HEPATITIS ASSOCIATED ANTIGEN OR ANTIBODY LABELED WITH THE RADIOACTIVE ISOTOPE 1-125;

    I. INCUBATING SAID WASHED TEST APPARATUS WHILE IN CONTACT WITH SAID PURIFIED FORM OF SAID HEPATITIS ASSOCIATED ANTIGEN OR ANTIBODY LABELED WITH A RADIOACTIVE ISOTOPE SO AS TO BOND SAID PURIFIED FORM TO SAID ANTIGEN OR ANTIBODY BONDED ON SAID TEST APPARATUS AND THEREBY PRODUCE A RADIOCATIVELY TRACTED INCUBATED COATING;

    J. WASHING SAID RADIOACTIVELY TRACED INCUBATED COATING WITH A BUFFERED SOLUTION TO REMOVE ANY UNBONDED PURIFIED FORM;

    K. COUNTING RADIATION EMITTED FROM SAID RADIOCATIVELY TRACTED INCUBATED COATING;

    AND L. COMPARING THE NUMBER OF COUNTS FROM SAID COATING WITH THE NUMBER OF COUNTS FROM A CONTROL SAMPLE PREPARED BY STEPS (A) TO (K).

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