RADIOIMMUNOASSAY FOR INSULIN

• US 3,867,518 A
• Filed: 03/09/1973
• Issued: 02/18/1975
• Est. Priority Date: 03/09/1973
• Status: Expired due to Term

• Current Assignee: Hans John Hansen, John William Coffey
• Original Assignee: Hans John Hansen, John William Coffey
• Inventors: Hansen, Hans John, Coffey, John William
• Primary Examiner(s): Padgett, Benjamin R.
• Application Number:
  US05/339,803
• Time in Patent Office: 711 Days
• Field of Search: 424/1 23/23B
• US Class Current: 436/539
• CPC Class Codes: G01N 33/74 involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
• CPC Class Codes: Y10S 436/815 Test for named compound or class of compounds
• CPC Class Codes: Y10S 436/817 Steroids or hormones

Abstract

A rapid radioimmunoassay for detecting immunoreactive insulin in body fluids, e.g., blood plasma, utilizing zirconyl phosphate gel at a pH at which free insulin will not bind to it, i.e., pH of 6.0 to 6.5, preferably 6.25, to adsorb antibody-bound insulin while leaving free insulin in solution, is disclosed.

Claims

1. IN A LIQUID PHASE RADIOIMMUNOASSAY FOR THE DETECTION OF IMMUNOREACTIVE INSULIN IN BLOOD PLASMA, THE IMPROVEMENT WHICH COMPRISES THE STEP OF ADDING A SLURRY OF ZIRCONYL PHOSPHATE GEL HAVING A PH OF FROM 6.0 TO 6.5 TO AN INCUBATION MIXTURE OF RADIOACTIVE INSULIN AND TEST FLUID TO STOP INCUBATION AND ADSORB ANTIBODY-BOUND INSULIN, SEPARATING THE ZIRCONYL PHOSPHATE GEL-BOUND INSULIN, SEPARATING THE ZIRCONYL RADIOCATIVITY THEREOF.

2. The radioimmunoassay of claim 1 wherein the pH of the zirconyl phosphate gel slurry is 6.25.

3. The radioimmunoassay of claim 1 wherein the zirconyl phosphate gel slurry contains on a volume basis about 40 percent to 45 percent solids.

4. The radioimmunoassay of claim 1 wherein the test fluid is blood plasma.