Determination of HCG glucose and galactose in body fluids with chromogenic indicators
First Claim
1. IN A PROCESS FOR THE DETECTION AND DETERMINATION OF A MEMBER OF THE GROUP CONSISTING OF HUMAN CHORIONIC GONADOTROPHIN, GLUCOSE, AND GALACTOSE PRESENT IN A FLUID SAMPLE, THE DETECTION AND DETERMINATION BEING BASED UPON A VISUAL COLOR CHANGE PRODUCED BY THE OXIDATION OF AN OXIDIZABLE CHROMOGENIC COMPOUND AS A COMPONENT IN A TEST REACTION SYSTEM INCLUDING OTHER TEST REACTION COMPONENTS, THE IMPROVEMENT WHICH COMPRISES INACTIVATING ANY UNDESIRED SUBSTANCES WHICH MAY BE PRESENT IN SAID FLUID SAMPLE BY FIRST CONTACTING THE SAMPLE WITH A PURIFIED CELLULOSE WHICH HAS BEEN FURTHER SUBJECTED TO TREATMENT WITH AN OXIDIZING AGENT, AND THEN CONTACTING THE SAMPLE WITH SAID CHROMOGENIC COMPOUND.
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Abstract
Process for the determination of a component of a reaction system, which determination is based upon the oxidation of a chromogenic compound and in which determination use is made of a test paper containing the remaining components of the reaction system. Such determinations are often disturbed by undesired compounds being present in the test fluid containing the component to be determined. The process is characterized in that the test fluid is first contacted with a material consisting essentially of cellulose or a cellulose-like substance, which material has been subjected to a treatment with an oxidizing agent. Test papers containing or made from the above-said material are useful for detecting HCG, glucose and galactose in body fluids.
18 Citations
5 Claims
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1. IN A PROCESS FOR THE DETECTION AND DETERMINATION OF A MEMBER OF THE GROUP CONSISTING OF HUMAN CHORIONIC GONADOTROPHIN, GLUCOSE, AND GALACTOSE PRESENT IN A FLUID SAMPLE, THE DETECTION AND DETERMINATION BEING BASED UPON A VISUAL COLOR CHANGE PRODUCED BY THE OXIDATION OF AN OXIDIZABLE CHROMOGENIC COMPOUND AS A COMPONENT IN A TEST REACTION SYSTEM INCLUDING OTHER TEST REACTION COMPONENTS, THE IMPROVEMENT WHICH COMPRISES INACTIVATING ANY UNDESIRED SUBSTANCES WHICH MAY BE PRESENT IN SAID FLUID SAMPLE BY FIRST CONTACTING THE SAMPLE WITH A PURIFIED CELLULOSE WHICH HAS BEEN FURTHER SUBJECTED TO TREATMENT WITH AN OXIDIZING AGENT, AND THEN CONTACTING THE SAMPLE WITH SAID CHROMOGENIC COMPOUND.
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2. The process of claim 1 in which said cellulose is in the form of a test paper.
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3. A test paper for the detection and determination of a member of the Group consisting of human chorionic gonadotrophin, glucose, and galactose in a fluid sample to be tested, the detection and determination being based upon a visual color change produced by the oxidation of an oxidizable chromogenic compound in a test reaction system including other test reaction components, said test paper consisting essentially of a purified cellulose which has been further subjected to treatment with an oxidizing agent, and is impregnated with said chromogenic compound in a reduced form and with said other test reaction components.
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4. A test paper for the detection and determination of human chorionic gonadotrophin in a fluid sample, the detection and determination being based upon a visual color change produced by the oxidation of an oxidizable chromogenic compound in a test reaction system including other test reaction components, said test paper consisting essentially of a purified cellulose which has been further subjected to treatment with an oxidizing agent, and being impregnated with said oxidizable chromogenic compound in a reduced form and with said remaining test reaction components including antibodies against human chorionic gonadotrophin, a conjugate of human chorionic gonadotrophin and peroxidase, insolubilized anti-antibodies, and a substrate for the peroxidase.
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5. A test paper for the detection and determination of human chorionic gonadotrophin in a fluid sample, the detection and determination being based upon a visual color change produced by the oxidation of an oxidizable chromogenic compound in a test reaction system including other test reaction components, said test paper consisting essentially of a purified cellulose which has been further subjected to treatment with an oxidizing agent, and being impregnated with said oxidizable chromogenic compound in a reduced form and with said remaining test reaction components including antibodies against human chorionic gonadotrophin, a conjugate of human chorionic gonadotrophin and a hydrogen peroxide-supplying oxido-reductase, a substrate for said oxido-reductase, insolubilized anti-antibodies, and peroxidase.
Specification