Medicament for the treatment of disorders of the eye

• US 3,869,548 A
• Filed: 01/22/1968
• Issued: 03/04/1975
• Est. Priority Date: 01/26/1967
• Status: Expired due to Term

• Current Assignee: Georges Dabis
• Original Assignee: Georges Dabis
• Inventors: Dabis, Georges
• Primary Examiner(s): Meyers, Albert T.
• Assistant Examiner(s): Ore, Dale R.
• Application Number:
  US04/699,293
• Time in Patent Office: 2,598 Days
• Field of Search: 424/98,94
• US Class Current: 424/94.67
• CPC Class Codes: A61K 35/58 Reptiles antigens from snakes A61K39/38

Abstract

Method of and medicament for the treatment of disorders of the eye, namely, diabetic retinopathy and degenerative maculation of the eye wherein doses containing heparin in combination with detoxified enzymes obtained from the venom of snakes, especially the Brazilian vipers Bothrops Jararaca and Lachesis Atrox, is administered, each dose containing 2000 to 2500 I.U. of heparin in accordance with the French Pharmacopoeia and 1 to 2 coagulation units (K) of the enzyme. The composition is administered parenterally.

Claims

1. A METHOD OF TREATING THE HUMAN EYE DISORDERS OF DIABETIC RETINOPATHY AND DEGENERATIVE MACULATION OF THE EYE, COMPRISING THE STEP OF ADMINISTERING BY MEANS OF INJECTION TO THE HUMAN A COMPOSITION CONTAINING HEPARIN AND A DETOXIFIED ENZYME OBTAINED FROM VENOM OF A BRAZILIAN VIPER SELECTED FROM THE GROUP CONSISTING OF BOTHROPS JARARACA AND LACHEIS ALTROX IN A DOSAGE OF 2000 TO 2500 INTERNATION UNITS OF HEPARIN IN ACCORANCE WITH THE FRENCH PHARMACOPEOIA AND 1 TO 2 COAGULATION UNITS OF THE ENZYME BEING OBTAINED BY D3DESIC AMILIORATED, THE DETOXIFIED ENZYME BEING OBTAINED BY DESICCATING THE VENOM TO FORM A VENOM POWDER, DISSOLVING THE VENOM POWDER IN DISTILLED WATER TO FORM A SOLUTION, ADDING AMMONIUM SULFATE CRYSTALS TO SAID SOLUTION TO PRECIPITATE AN ENZYMATICALLY ACTIVE FIRST FRACTION, RECOVERING SAID ENZYMATICALLY ACTIVE FIRST FRACTION FROM THE SOLUTION BY FILTRATION, PRECIPITATING FROM THE FILTRATE BY ADDITION OF MAGNESIUM SULFATE THERETO TO FORM AN ENZYMATICALLY TOXIC SECOND FRACTION, PURIFYING THE FRACTIONS BY DIALYSIS, DETOXIFYING THE SECOND FRACTION BY EXPOSING IT TO THE LIGHT OF A QUARTZ LAMP, MIXING THE FRACTIONS TO OBTAIN AN ENZYMATICALLY ACTIVE PRODUCT OF CONSTANT AND DEFINATIVE ACTIVITY, AND MIXING SAID PRODUCT WITH HEPARIN.

2. A medicament for the treatment of the human eye disorders diabetic retinopathy and degenerative maculation which consists essentially of heparin and a detoxified enzyme obtained from venom of a Brazilian viper selected from the group consisting of Bothrops Jararaca and Lachesis Atrox in a dosage of 2000 to 2500 International Units of heparin in accordance with the French Pharmacopoeia and 1 to 2 coagulation units of the enzyme, the detoxified enzyme being obtained by desiccating the venom to form a venom powder, dissolving the venom powder in distilled water to form a solution, adding ammonium sulate crystals to said solution to precipitate an enzymatically active first fraction, recovering said enzymatic first fraction from the solution by filtration, precipitating from the filtrate by addition of magnesium sulfate thereto to form an enzymatically toxic second fraction, purifying the fractions by dialysis, detoxifying the second fraction by exposing it to the light of a quartz lamp, mixing the fractions to obtain an enzymatically active product of constant and definitive activity, and mixing said product with heparin.

3. A method of making a medicament, comprisisng the steps of obtaining a venom from a Brazilian vipEr selected from the group consisting of Bothrops Jararaca and Lachesis Atrox, desiccating the venom to form a venom powder, dissolving the venom powder in distilled water to form a solution adding ammonium sulfate crystals to said solution to precipitate an enzymatically active first fraction, recovering said enzymatic first fraction from the solution by filtration, precipitating from the filtrate by addition of magnesium sulfate thereto to form an enzymatically toxic second fraction, purifying the fractions by dialysis, detoxifying the second fraction by exposing it to the light of a quartz lamp, mixing the fractions to obtain an enzymatically active product of constant and definitive activity, and mixing said product with heparin in a proportion of about 1 to 2 coagulation units (K) of the product per 2000 to 2500 I.U. of heparin.