TEST FOR METABOLIC CONDITIONS IN BLOOD OR SERUM

• US 3,877,873 A
• Filed: 06/26/1972
• Issued: 04/15/1975
• Est. Priority Date: 06/26/1972
• Status: Expired due to Term

• Current Assignee: Milton Winitz
• Original Assignee: Milton Winitz
• Inventors: Winitz, Milton
• Primary Examiner(s): Scovronek, Joseph
• Assistant Examiner(s): Turk, Arnold
• Application Number:
  US05/266,087
• Time in Patent Office: 1,023 Days
• Field of Search: 23/23B 252/408
• US Class Current: 436/108
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72
• CPC Class Codes: Y10T 436/171538 Urea or blood urea nitrogen

Abstract

Simple inexpensive testing procedures are provided for detecting the existence of a large number of different diseased metabolic states whereby whole blood or blood serum is added to reagent solutions and the resulting color development or opacity compared to known standards. Depending upon the diseased state, gentian violet (methylrosaniline chloride) or methyl red (pdimethylaminoazobenzene-o-carboxylic acid) is employed as a colorant in at least one reagent solution in dilute saline water. Usually, there is one other reagent solution of distilled water (substantially free of salt). For determining certain diseased states, such as leukemia, Hodgkin'"'"''"'"'s disease, sickle cell anemia or the trait, and alkalosis, heparin is also included in at least one reagent solution. For respiratory acidosis, metabolic acidosis, hypoglycemia and hyperglycemia, heparin is not employed and only distilled and saline solution with gentian violet are employed as the reagents.

Claims

1. A COLORIMETRIC METHOD FOR DETERMINING THE METABOLIC DISEASE STATES:

GRANULOCYTIC LEUKEMIA, LYMPHOCYTIC LEUKEMIA, MONOCYTIC LEUKEMIA, PRIMITIVE WHITE CELL LEUKEMIA, LYMPHOSARCOMA, HODGKINS'"'"''"'"'S DESEASE, SICKLE-CELL ANEMIA OR TRAIT, RESPIRATORY ACIDOSIS, METABOLIC ACIDOSIS, HYPOGLYCEMIA AND HYPERGLYCEMIA, WHICH COMPRISES FORMING AT LEAST ONE DILUTE SOLUTION OF WHOLE BLOOD SERUM IN DILUTE SALINE IN THE PRESENCE OF GENTIASR179SR179AND COMPARING THE COLOR PRODUCED TO A STANDARD.

2. A method according to claim 1 for determining Hodgkin'"'"''"'"'s disease which comprises diluting whole blood into distilled water;

introducing an aliquot of the diluted whole blood into a dilute solution containing heparin;

transferring an aliquot of said heparin solution into a dilute solution of gentian violet reagent in distilled water;

transferring an aliquot from said gentian violet solution to a dilute saline solution and repeating the transfers to successive saline solutions; and

comparing the development of color to known standards.

3. A method according to claim 1 for determining sickle cell anemia or trait which comprises diluting whole blood in distilled water;

transferring an aliquot of said diluted whole blood solution to saline solution;

transferring an aliquot of said saline solution to saline solution containing gentian violet reagent; and

transferring an aliquot of said gentian violet saline solution to a saline solution containing gentian violet and heparin; and

comparing the resulting colors to known standards.

4. A method according to claim 1 for determining acidosis or glycemia which comprises introducing into a first tube containing dilute saline solution and gentian violet reagent and a second tube containing distilled water and dilute gentian violet reagent blood serum;

and comparing the resulting colors to known standards.

5. A method according to claim 1 wherein a small amount of heparin is included in said solution.

6. A method according to claim 1 for determining different types of leukemia which comprises introducing into a first solution of saline containing gentian violet reagent, a small amount of blood serum;

introducing into a second solution of distilled water containing gentian violet reagent a small amount of blood serum;

transferring an aliquot from said distilled water solution to a third solution of dilute heparin solution of dilute saline; and

comparing the colors produced to known standards.

7. A method according to claim 6, which includes having at least one additional saline solution and transferring an aliquot from said third solution to a fourth solution of saline, and from said fourth and succeeding solution of saline, transferring an aliquot from a solution which has previously received an aliquot to the next succeeding solution of saline.

8. A method for determining alkalosis which comprises introducing into a first solution of saline containing methyl red reagent, a small amount of blood serum;

introducing into a second solution of distilled water containing methyl red reagent a small amount of blood serum;

transferring an aliquot from said distilled water solution to a third solution of dilute heparin solution of dilute saline; and

comparing the colors produced to known standards.