Method of producing L-arginine by fermentation
First Claim
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1. A METHOD OF PRODUCING L-ARGININE WHICH COMPRISES:
- A. CULTURING BREVIBACTERIUM FLAVUN FERM-P 1639 IN AN AQUEOUS MEDIUM CONTAINING ASSIMILABLE SOURCES OF CARBON AND NITROGEN, INORGANIC SALTS, AND MINOR ORGANIC NUTRIENTS NECESSARY FOR GROWTH OF SAID BREVIBACTERIUM FLAVUM UNTIL ARGININE ACCUMULATES IN SAID MEDIUM;
AND B. RECOVERING THE ACCUMULATED ARGININE.
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Abstract
An artificially induced mutant strain of Brevibacterium flavum which requires guanine for its growth and is capable of growth in a medium containing 10 mg/ml 2-thiazolalanine or other arginine analogs produces L-arginine in high yields from carbohydrates, acetate ions, or ethanol as carbon sources.
9 Citations
2 Claims
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1. A METHOD OF PRODUCING L-ARGININE WHICH COMPRISES:
- A. CULTURING BREVIBACTERIUM FLAVUN FERM-P 1639 IN AN AQUEOUS MEDIUM CONTAINING ASSIMILABLE SOURCES OF CARBON AND NITROGEN, INORGANIC SALTS, AND MINOR ORGANIC NUTRIENTS NECESSARY FOR GROWTH OF SAID BREVIBACTERIUM FLAVUM UNTIL ARGININE ACCUMULATES IN SAID MEDIUM;
AND B. RECOVERING THE ACCUMULATED ARGININE.
- A. CULTURING BREVIBACTERIUM FLAVUN FERM-P 1639 IN AN AQUEOUS MEDIUM CONTAINING ASSIMILABLE SOURCES OF CARBON AND NITROGEN, INORGANIC SALTS, AND MINOR ORGANIC NUTRIENTS NECESSARY FOR GROWTH OF SAID BREVIBACTERIUM FLAVUM UNTIL ARGININE ACCUMULATES IN SAID MEDIUM;
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2. A method as set forth in claim 1, wherein said assimilable source of carbon is at least one member of the group consisting of carbohydrate, acetate ions, and ethanol.
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Current AssigneeHirotaka Kamijo, Koji Kubota, Shinji Okumura, Takiko Onoda
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Original AssigneeHirotaka Kamijo, Koji Kubota, Shinji Okumura, Takiko Onoda
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InventorsKubota, Koji, Onoda, Takiko, Okumura, Shinji, Kamijo, Hirotaka
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Primary Examiner(s)Shapiro, Lionel M.
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Assistant Examiner(s)Warden, Robert J.
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Application NumberUS05/402,020Time in Patent Office561 DaysField of Search195/28,29,30US Class Current435/114CPC Class CodesC12P 13/10 Citrulline; Arginine; Ornit...Y10S 435/84 Brevibacterium
