Culture medium for tissue culture techniques
First Claim
1. In a tissue culture method comprising the steps of inoculating a protein-free, sterile tissue culture medium with living animal tissue cells and incubating said inoculated tissue culture medium under conditions conductive to cell propagation, the improvement which comprises the steps of mixing a growth-enhancing amount of a water-soluble lipid source selected from the group consisting of polysorbate 60 and polysorbate 80 with said medium, in an amount of from about 0.0010 to about 0.100 part by volume of lipid source per 100 parts by volume of medium, intimately contacting said medium with a growth-enhancing amount of a strongly basic anion-exhance resin obtained by reacting trimethylamine with a halomethylated polymer prepared by reacting chloromethyl methyl ether in the presence of a zinc chloride catalyst with beads of a copolymer of about 98 percent of styrene and 2 percent of divinylbenzene and thereafter incubating said tissue culture medium, cells and polysorbate lipid source in intimate contact with said strongly basic anion-exchange resin under conditions conducive to cell propagation for a time sufficient to obtain substantial propagation of said cells.
0 Assignments
0 Petitions
Accused Products
Abstract
A chemically defined, protein-free culture medium embodying an anion-exchange resin and a water-soluble lipid source provides means for tissue culture.
-
Citations
2 Claims
-
1. In a tissue culture method comprising the steps of inoculating a protein-free, sterile tissue culture medium with living animal tissue cells and incubating said inoculated tissue culture medium under conditions conductive to cell propagation, the improvement which comprises the steps of mixing a growth-enhancing amount of a water-soluble lipid source selected from the group consisting of polysorbate 60 and polysorbate 80 with said medium, in an amount of from about 0.0010 to about 0.100 part by volume of lipid source per 100 parts by volume of medium, intimately contacting said medium with a growth-enhancing amount of a strongly basic anion-exhance resin obtained by reacting trimethylamine with a halomethylated polymer prepared by reacting chloromethyl methyl ether in the presence of a zinc chloride catalyst with beads of a copolymer of about 98 percent of styrene and 2 percent of divinylbenzene and thereafter incubating said tissue culture medium, cells and polysorbate lipid source in intimate contact with said strongly basic anion-exchange resin under conditions conducive to cell propagation for a time sufficient to obtain substantial propagation of said cells.
-
2. The method of claim 1 wherein the lipid source is polysorbate 60. 80.
Specification
-
- Resources
-
Current AssigneeDale E. Bordt, Harry L. Torney, Helen T. Torney
-
Original AssigneeDale E. Bordt, Harry L. Torney, Helen T. Torney
-
InventorsTorney, Harry L., Torney, Helen T., Bordt, Dale E.
-
Primary Examiner(s)Huff, Richard L.
-
Application NumberUS05/377,838Time in Patent Office694 DaysField of Search195/1.7US Class Current435/396CPC Class CodesC12N 2500/36 LipidsC12N 2500/50 Soluble polymers, e.g. poly...C12N 5/005 Protein-free medium
