Flat plate electrophoresis

• US 3,888,759 A
• Filed: 05/25/1973
• Issued: 06/10/1975
• Est. Priority Date: 05/25/1973
• Status: Expired due to Term

• Current Assignee: David Elson, Shlomo Avital
• Original Assignee: David Elson, Shlomo Avital
• Inventors: Elson, David, Avital, Shlomo
• Primary Examiner(s): Mack, John H.
• Assistant Examiner(s): Prescott, A. C.
• Application Number:
  US05/363,961
• Time in Patent Office: 746 Days
• Field of Search: 204/18G,18S,299
• US Class Current: 204/620
• CPC Class Codes: G01N 27/44756 Apparatus specially adapted therefor
• CPC Class Codes: G01N 27/44773 Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Abstract

A device for horizontal flat plate electrophoresis comprising in combination a horizontal plate defining a rectangular gel bed, surrounded by a ledge, the height of which defines the height of the gel, and by lateral walls, a cover adapted to rest on the ledge, fixing the thickness of the gel, two legs attached to the bottom of the plate and extennding along the entire width of the plate at each end, a slot being provided at each end of the gel bed and running the width of same said slots extending through the entire height of the leg, the cover being provided with a plurality of holes for the insertion of templates during the polymerization of the gel, and for the subsequent insertion of the samples. The gel bed can be divided into a plurality of separate channels, and openings for the insertion of samples are provided near the edge of the cover or at about its middle.

Claims

1. A DEVICE FOR HORIZONTAL FLAT PLATE ELECTROPHORESIS COMPRISING IN COMBINATION, A HORIZONTAL PLATE HAVING AN UPPER AND A LOWER SURFACE AND SURROUNDED BY LATERAL WALLS, THE UPPER SURFACE OF THE PLATE AND THE LATERAL WALLS DEFINING A RECTANGULAR GEL BED;

A STEP OF PREDETERMINED HEIGHT IN EACH OF THE LATERAL WALLS THEREBY DEFINING A LEDGE;

A COVER ADAPTED TO REST ON THE LEDGE AND THEREBY DEFINE THE THICKNESS OF THE GEL BED;

A PLURALITY OF HOLES IN THE COVER FOR THE INSERTION OF TEMPLATES DURING POLYMERIZATION OF THE GEL TO DEFINE PREDETERMINED HOLES OF PREDETERMINED VOLUME IN THE GEL AND FOR INSERTION OF SAMPLES OF PREDETERMINED VOLUME IN THE HOLES;

TWO LEGS ATTACHED TO THE LOWER SURFACE OF THE PLATE AT OPPOSING SIDES THEREOF AND COEXTENSIVE WITH THE SIDES TO WHICH THE LEGS ARE ATTACHED;

AND A SLOT THROUGH THE PLATE POSITIONED OVER EACH LEG, EXTENDING FOR THE FULL LENGTH OF THE LEG, AND EXTENDING THROUGH THE ENTIRE HEIGHT OF THE LEG.

2. The device of claim 1 wherein the horizontal plate defines a square gel bed.

3. The device of claim 2 wherein the gel bed is divided into a plurality of separate channels, defining individual gel strips, separate from each other.

4. The device of claim 2 wherein the holes in the cover for the insertion of templates are located near one of the edges of the cover and about the middle of the cover.

5. The device of claim 2 further comprising templates of varying predetermined volume so as to define in the gel bed space for predetermined vaying samples of predetermined varying volume.

6. The device of claim 2 wherein the gel bed is divided into a plurality of separate channels, defining individual gel strips, separate from each other, and wherein two of the holes in the cover are located above the same channel, one of which is positioned near one of the slots and the other hole is offset toward the center of the cover.

7. The device of claim 6 further comprising templates in each of the two holes, each of the templates being of a different shape.