Fluorescent derivatives of cytosine-containing compounds

• US 3,893,998 A
• Filed: 02/22/1973
• Issued: 07/08/1975
• Est. Priority Date: 02/22/1973
• Status: Expired due to Term

• Current Assignee: John A. Secrist III, Jorge R. Barrio, Laurence G. Dammann, Nelson J. Leonard
• Original Assignee: John A. Secrist III, Jorge R. Barrio, Laurence G. Dammann, Nelson J. Leonard
• Inventors: Secrist, John A. III, Barrio, Jorge R., Leonard, Nelson J., Dammann, Laurence G.
• Primary Examiner(s): Brown, Johnnie R.
• Application Number:
  US05/334,674
• Time in Patent Office: 866 Days
• Field of Search: 260/211.5R
• US Class Current: 536/26.6
• CPC Class Codes: C07H 19/06 Pyrimidine radicals
• CPC Class Codes: C07H 19/10 with the saccharide radical esterified by phosphoric or polyphosphoric acids

Abstract

Fluorescent analogs of biologically active coenzymes are made by reaction of certain cytosine-containing coenzymes with chloroacetaldehyde. The reaction products have improved biologic activity, and have improved utility for the investigation of enzyme processes.

Claims

1. A BIOLOGICALLY ACTIVE FLUORESCENT COENZYME SLECTED FROM THE GROUP CONSISTING OF:

A. COMPOUNDS HAVING THE FORMULA

2. 3,N4-ethenoarabinosylcytosine.

3. 3,N4-ethenocytidine 3'"'"''"'"'-monophosphate.

4. 3,N4-ethenocytidine 5'"'"''"'"'-monophosphate.

5. 3,N4-ethenocytidine 5'"'"''"'"'-diphosphate.

6. 3,N4-ethenocytidine 5'"'"''"'"'-triphosphate.

7. 3,N4-ethenocytidine 5'"'"''"'"'- gamma -32P-triphosphate.

8. 3,N4-ethenocytidine 3'"'"''"'"', 5'"'"''"'"'-cyclic-monophosphate.